Universität zu Köln

Saebelfeld, Manja, Das, Suman G., Brink, Jorn, Hagenbeek, Arno, Krug, Joachim and de Visser, J. Arjan G. M. (2021). Antibiotic Breakdown by Susceptible Bacteria Enhances the Establishment of beta-Lactam Resistant Mutants. Front. Microbiol., 12. LAUSANNE: FRONTIERS MEDIA SA. ISSN 1664-302X

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Abstract

For a better understanding of the evolution of antibiotic resistance, it is imperative to study the factors that determine the initial establishment of mutant resistance alleles. In addition to the antibiotic concentration, the establishment of resistance alleles may be affected by interactions with the surrounding susceptible cells from which they derive, for instance via the release of nutrients or removal of the antibiotic. Here, we investigate the effects of social interactions with surrounding susceptible cells on the establishment of Escherichia coli mutants with increasing beta-lactamase activity (i.e., the capacity to hydrolyze beta-lactam antibiotics) from single cells under the exposure of the antibiotic cefotaxime (CTX) on agar plates. We find that relatively susceptible cells, expressing a beta-lactamase with very low antibiotic-hydrolyzing activity, increase the probability of mutant cells to survive and outgrow into colonies due to the active breakdown of the antibiotic. However, the rate of breakdown by the susceptible strain is much higher than expected based on its low enzymatic activity. A detailed theoretical model suggests that this observation may be explained by cell filamentation causing delayed lysis. While susceptible cells may hamper the spread of higher-resistant beta-lactamase mutants at relatively high frequencies, our findings show that they promote their initial establishment.</p>

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Saebelfeld, Manja UNSPECIFIED UNSPECIFIED UNSPECIFIED Das, Suman G. UNSPECIFIED UNSPECIFIED UNSPECIFIED Brink, Jorn UNSPECIFIED UNSPECIFIED UNSPECIFIED Hagenbeek, Arno UNSPECIFIED UNSPECIFIED UNSPECIFIED Krug, Joachim UNSPECIFIED UNSPECIFIED UNSPECIFIED de Visser, J. Arjan G. M. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-574994
DOI:	10.3389/fmicb.2021.698970
Journal or Publication Title:	Front. Microbiol.
Volume:	12
Date:	2021
Publisher:	FRONTIERS MEDIA SA
Place of Publication:	LAUSANNE
ISSN:	1664-302X
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language CELL; EVOLUTION; DYNAMICS; SPECTRUM; GROWTH; DEATH Multiple languages Microbiology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/57499