Universität zu Köln

Hou, Anwei, Goldfuss, Bernd and Dickschat, Jeroen S. (2021). Functional Switch and Ethyl Group Formation in the Bacterial Polytrichastrene Synthase from Chryseobacterium polytrichastri. Angew. Chem.-Int. Edit., 60 (38). S. 20781 - 20786. WEINHEIM: WILEY-V C H VERLAG GMBH. ISSN 1521-3773

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Abstract

A reinvestigation of the linalool synthase from Chryseobacterium polytrichastri uncovered its diterpene synthase activity, yielding polytrichastrene A and polytrichastrol A with new skeletons, besides known wanju-2,5-diene and thunbergol. The enzyme mechanism was investigated by isotopic labeling experiments and DFT calculations to explain an unusual ethyl group formation. Rationally designed exchanges of active site residues showed major functional switches, resulting for I66F in the production of five more new compounds, including polytrichastrene B and polytrichastrol B, while A87T, A192V and the double exchange A87T, A192V gave a product shift towards wanju-2,5-diene.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Hou, Anwei UNSPECIFIED UNSPECIFIED UNSPECIFIED Goldfuss, Bernd UNSPECIFIED UNSPECIFIED UNSPECIFIED Dickschat, Jeroen S. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-575006
DOI:	10.1002/anie.202109465
Journal or Publication Title:	Angew. Chem.-Int. Edit.
Volume:	60
Number:	38
Page Range:	S. 20781 - 20786
Date:	2021
Publisher:	WILEY-V C H VERLAG GMBH
Place of Publication:	WEINHEIM
ISSN:	1521-3773
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language MOLECULAR-CLONING; TERPENE SYNTHASES; SESQUITERPENE; GENOME; EXPRESSION; CYCLASE; STEREOCHEMISTRY; BIOSYNTHESIS; CYCLIZATION; CHEMISTRY Multiple languages Chemistry, Multidisciplinary Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/57500