Universität zu Köln

Blaum, Franziska, Lukas, Dominika, Reddehase, Matthias J. and Lemmermann, Niels A. W. ORCID: 0000-0001-9190-6497 (2021). Localization of Viral Epitope-Specific CD8 T Cells during Cytomegalovirus Latency in the Lungs and Recruitment to Lung Parenchyma by Airway Challenge Infection. Life-Basel, 11 (9). BASEL: MDPI. ISSN 2075-1729

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Abstract

Interstitial pneumonia is a life-threatening clinical manifestation of cytomegalovirus infection in recipients of hematopoietic cell transplantation (HCT). The mouse model of experimental HCT and infection with murine cytomegalovirus revealed that reconstitution of virus-specific CD8(+) T cells is critical for resolving productive lung infection. CD8(+) T-cell infiltrates persisted in the lungs after the establishment of latent infection. A subset defined by the phenotype KLRG1(+)CD62L(-) expanded over time, a phenomenon known as memory inflation (MI). Here we studied the localization of these inflationary T effector-memory cells (iTEM) by comparing their frequencies in the intravascular and transmigration compartments, the IVC and TMC, respectively, with their frequency in the extravascular compartment (EVC), the alveolar epithelium. Frequencies of viral epitope-specific iTEM were comparable in the IVC and TMC but were reduced in the EVC, corresponding to an increase in KLRG1(-)CD62L(-) conventional T effector-memory cells (cTEM) and a decrease in functional IFN gamma(+)CD8(+) T cells. As maintained expression of KLRG1 requires stimulation by antigen, we conclude that iTEM lose KLRG1 and convert to cTEM after transmigration into the EVC because pneumocytes are not latently infected and, therefore, do not express antigens. Accordingly, antigen re-expression upon airway challenge infection recruited virus-specific CD8(+) T cells to TMC and EVC.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Blaum, Franziska UNSPECIFIED UNSPECIFIED UNSPECIFIED Lukas, Dominika UNSPECIFIED UNSPECIFIED UNSPECIFIED Reddehase, Matthias J. UNSPECIFIED UNSPECIFIED UNSPECIFIED Lemmermann, Niels A. W. UNSPECIFIED orcid.org/0000-0001-9190-6497 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-576139
DOI:	10.3390/life11090918
Journal or Publication Title:	Life-Basel
Volume:	11
Number:	9
Date:	2021
Publisher:	MDPI
Place of Publication:	BASEL
ISSN:	2075-1729
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language MEMORY INFLATION; HERPESVIRUS GENOME; PERSISTENCE; MECHANISMS; HISTORY Multiple languages Biology; Microbiology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/57613