Universität zu Köln

Arshaad, Muhammad Imran, Siwek, Magdalena Elisabeth, Henseler, Christina, Daubner, Johanna, Ehninger, Dan, Hescheler, Juergen, Sachinidis, Agapios, Broich, Karl, Papazoglou, Anna and Weiergraeber, Marco (2021). Enhanced hippocampal type II theta activity AND altered theta architecture in mice lacking the Ca(v)3.2 T-type voltage-gated calcium channel. Sci Rep, 11 (1). BERLIN: NATURE PORTFOLIO. ISSN 2045-2322

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Abstract

T-type Ca2+ channels are assumed to contribute to hippocampal theta oscillations. We used implantable video-EEG radiotelemetry and qPCR to unravel the role of Ca(v)3.2 Ca2+ channels in hippocampal theta genesis. Frequency analysis of spontaneous long-term recordings in controls and Ca(v)3.2(-/-) mice revealed robust increase in relative power in the theta (4-8 Hz) and theta-alpha (4-12 Hz) ranges, which was most prominent during the inactive stages of the dark cycles. Urethane injection experiments also showed enhanced type II theta activity and altered theta architecture following Ca(v)3.2 ablation. Next, gene candidates from hippocampal transcriptome analysis of control and Ca(v)3.2(-/-) mice were evaluated using qPCR. Dynein light chain Tctex-Type 1 (Dynlt1b) was significantly reduced in Ca(v)3.2(-/-) mice. Furthermore, a significant reduction of GABA A receptor delta subunits and GABA B1 receptor subunits was observed in the septohippocampal GABAergic system. Our results demonstrate that ablation of Ca(v)3.2 significantly alters type II theta activity and theta architecture. Transcriptional changes in synaptic transporter proteins and GABA receptors might be functionally linked to the electrophysiological phenotype.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Arshaad, Muhammad Imran UNSPECIFIED UNSPECIFIED UNSPECIFIED Siwek, Magdalena Elisabeth UNSPECIFIED UNSPECIFIED UNSPECIFIED Henseler, Christina UNSPECIFIED UNSPECIFIED UNSPECIFIED Daubner, Johanna UNSPECIFIED UNSPECIFIED UNSPECIFIED Ehninger, Dan UNSPECIFIED UNSPECIFIED UNSPECIFIED Hescheler, Juergen UNSPECIFIED UNSPECIFIED UNSPECIFIED Sachinidis, Agapios UNSPECIFIED UNSPECIFIED UNSPECIFIED Broich, Karl UNSPECIFIED UNSPECIFIED UNSPECIFIED Papazoglou, Anna UNSPECIFIED UNSPECIFIED UNSPECIFIED Weiergraeber, Marco UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-578409
DOI:	10.1038/s41598-020-79763-4
Journal or Publication Title:	Sci Rep
Volume:	11
Number:	1
Date:	2021
Publisher:	NATURE PORTFOLIO
Place of Publication:	BERLIN
ISSN:	2045-2322
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language GABA(A) RECEPTOR TRAFFICKING; CA(V)2.3 R-TYPE; CA2+ CHANNELS; INTERNATIONAL UNION; GABAERGIC NEURONS; MEDIAL SEPTUM; DYNAMIC MODULATION; ION CHANNELS; PHARMACOLOGY; OSCILLATIONS Multiple languages Multidisciplinary Sciences Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/57840