Universität zu Köln

Muller, Lisa, Ostermann, Philipp Niklas ORCID: 0000-0002-4419-6119, Walker, Andreas, Wienemann, Tobias ORCID: 0000-0002-1157-8700, Mertens, Alexander, Adams, Ortwin, Andree, Marcel, Hauka, Sandra, Lubke, Nadine, Keitel, Verena ORCID: 0000-0003-1383-7662, Drexler, Ingo, Di Cristanziano, Veronica, Hermsen, Derik Franz, Kaiser, Rolf, Boege, Friedrich, Klein, Florian, Schaal, Heiner, Timm, Joerg and Senff, Tina ORCID: 0000-0003-1578-8163 (2021). Sensitivity of anti-SARS-CoV-2 serological assays in a high-prevalence setting. Eur. J. Clin. Microbiol. Infect. Dis., 40 (5). S. 1063 - 1072. NEW YORK: SPRINGER. ISSN 1435-4373

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Abstract

Evaluation and power of seroprevalence studies depend on the performed serological assays. The aim of this study was to assess four commercial serological tests from EUROIMMUN, DiaSorin, Abbott, and Roche as well as an in-house immunofluorescence and neutralization test for their capability to identify SARS-CoV-2 seropositive individuals in a high-prevalence setting. Therefore, 42 social and working contacts of a German super-spreader were tested. Consistent with a high-prevalence setting, 26 of 42 were SARS-CoV-2 seropositive by neutralization test (NT), and immunofluorescence test (IFT) confirmed 23 of these 26 positive test results (NT 61.9% and IFT 54.8% seroprevalence). Four commercial assays detected anti-SARS-CoV-2 antibodies in 33.3-40.5% individuals. Besides an overall discrepancy between the NT and the commercial assays regarding their sensitivity, this study revealed that commercial SARS-CoV-2 spike-based assays are better to predict the neutralization titer than nucleoprotein-based assays are.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Muller, Lisa UNSPECIFIED UNSPECIFIED UNSPECIFIED Ostermann, Philipp Niklas UNSPECIFIED orcid.org/0000-0002-4419-6119 UNSPECIFIED Walker, Andreas UNSPECIFIED UNSPECIFIED UNSPECIFIED Wienemann, Tobias UNSPECIFIED orcid.org/0000-0002-1157-8700 UNSPECIFIED Mertens, Alexander UNSPECIFIED UNSPECIFIED UNSPECIFIED Adams, Ortwin UNSPECIFIED UNSPECIFIED UNSPECIFIED Andree, Marcel UNSPECIFIED UNSPECIFIED UNSPECIFIED Hauka, Sandra UNSPECIFIED UNSPECIFIED UNSPECIFIED Lubke, Nadine UNSPECIFIED UNSPECIFIED UNSPECIFIED Keitel, Verena UNSPECIFIED orcid.org/0000-0003-1383-7662 UNSPECIFIED Drexler, Ingo UNSPECIFIED UNSPECIFIED UNSPECIFIED Di Cristanziano, Veronica UNSPECIFIED UNSPECIFIED UNSPECIFIED Hermsen, Derik Franz UNSPECIFIED UNSPECIFIED UNSPECIFIED Kaiser, Rolf UNSPECIFIED UNSPECIFIED UNSPECIFIED Boege, Friedrich UNSPECIFIED UNSPECIFIED UNSPECIFIED Klein, Florian UNSPECIFIED UNSPECIFIED UNSPECIFIED Schaal, Heiner UNSPECIFIED UNSPECIFIED UNSPECIFIED Timm, Joerg UNSPECIFIED UNSPECIFIED UNSPECIFIED Senff, Tina UNSPECIFIED orcid.org/0000-0003-1578-8163 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-579848
DOI:	10.1007/s10096-021-04169-7
Journal or Publication Title:	Eur. J. Clin. Microbiol. Infect. Dis.
Volume:	40
Number:	5
Page Range:	S. 1063 - 1072
Date:	2021
Publisher:	SPRINGER
Place of Publication:	NEW YORK
ISSN:	1435-4373
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language Infectious Diseases; Microbiology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/57984