Universität zu Köln

Hellmich, Luisa, Witte, Kai-Erik, Ebinger, Martin ORCID: 0000-0002-4229-8058 and Ulmer, Anja (2021). Flow Cytometry for Detection and Quantification of Micrometastases in Sentinel Lymph Nodes from Patients with Primary Melanoma. J. Surg. Res., 257. S. 477 - 486. SAN DIEGO: ACADEMIC PRESS INC ELSEVIER SCIENCE. ISSN 1095-8673

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Abstract

Background: Detection of micrometastases in the regional lymph nodes is one of the most important prognostic factors for melanoma patients. Our aim was to evaluate the suitability of flow cytometry for rapid quantification of disseminated melanoma cells in sentinel lymph nodes (SLN). Methods: 132 SLNs from 104 patients diagnosed with melanoma were analyzed by flow cytometry, utilizing the extracellular marker melanoma-associated chondroitin sulfate proteoglycan, in addition to quantitative immunocytology and conventional histopathology, including immunohistochemistry. For quantification, the number of melanoma positive cells per million lymph node cells (disseminated cancer cell density, DCCD) detected by flow cytometry was compared to the DCCD obtained by immunocytology. Results: Compared to histopathology and immunocytology, flow cytometry exhibited a sensitivity of 50% and a specificity of 85%. DCCDs of immunocytology and flow cytometry of the 37 immunocytologically positive SLNs showed a positive correlation (Spearman's rho = 0.7,P < 0.0001). In 10 SLNs from 9 patients with high tumor load, the flow cytometric DCCD was 8-fold higher on average than the immunocytologic DCCD. Conclusions: Although flow cytometry is not yet suitable for early detection of metastatic melanoma, it promises to become a valuable tool for rapidly quantifying tumor load in high-risk patients. (c) 2020 Elsevier Inc. All rights reserved.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Hellmich, Luisa UNSPECIFIED UNSPECIFIED UNSPECIFIED Witte, Kai-Erik UNSPECIFIED UNSPECIFIED UNSPECIFIED Ebinger, Martin UNSPECIFIED orcid.org/0000-0002-4229-8058 UNSPECIFIED Ulmer, Anja UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-581683
DOI:	10.1016/j.jss.2020.08.028
Journal or Publication Title:	J. Surg. Res.
Volume:	257
Page Range:	S. 477 - 486
Date:	2021
Publisher:	ACADEMIC PRESS INC ELSEVIER SCIENCE
Place of Publication:	SAN DIEGO
ISSN:	1095-8673
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language HISTOPATHOLOGIC EVALUATION; PROGNOSTIC-SIGNIFICANCE; MALIGNANT-MELANOMA; STAGE-III; CELLS; DEPRIVATION; METASTASIS; DISSECTION; SERUM Multiple languages Surgery Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/58168