Universität zu Köln

Allmeroth, Kira ORCID: 0000-0002-2659-6776, Kim, Christine S., Annibal, Andrea ORCID: 0000-0003-4311-5417, Pouikli, Andromachi ORCID: 0000-0003-1868-6875, Koester, Janis ORCID: 0000-0002-5274-0591, Derisbourg, Maxime J., Chacon-Martinez, Carlos Andres, Latza, Christian, Antebi, Adam, Tessarz, Peter ORCID: 0000-0002-6953-9835, Wickstroem, Sara A. and Denzel, Martin S. (2021). N1-acetylspermidine is a determinant of hair follicle stem cell fate. J. Cell Sci., 134 (9). CAMBRIDGE: COMPANY BIOLOGISTS LTD. ISSN 1477-9137

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Abstract

Stem cell differentiation is accompanied by increased mRNA translation. The rate of protein biosynthesis is influenced by the polyamines putrescine, spermidine and spermine, which are essential for cell growth and stem cell maintenance. However, the role of polyamines as endogenous effectors of stem cell fate and whether they act through translational control remains obscure. Here, we investigate the function of polyamines in stem cell fate decisions using hair follicle stem cell (HFSC) organoids. Compared to progenitor cells, HFSCs showed lower translation rates, correlating with reduced polyamine levels. Surprisingly, overall polyamine depletion decreased translation but did not affect cell fate. In contrast, specific depletion of natural polyamines mediated by spermidine/spermine N1-acetyltransferase (SSAT; also known as SAT1) activation did not reduce translation but enhanced stemness. These results suggest a translation-independent role of polyamines in cell fate regulation. Indeed, we identified N1-acetylspermidine as a determinant of cell fate that acted through increasing self-renewal, and observed elevated N1-acetylspermidine levels upon depilation-mediated HFSC proliferation and differentiation in vivo. Overall, this study delineates the diverse routes of polyamine metabolism-mediated regulation of stem cell fate decisions. This article has an associated First Person interview with the first author of the paper.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Allmeroth, Kira UNSPECIFIED orcid.org/0000-0002-2659-6776 UNSPECIFIED Kim, Christine S. UNSPECIFIED UNSPECIFIED UNSPECIFIED Annibal, Andrea UNSPECIFIED orcid.org/0000-0003-4311-5417 UNSPECIFIED Pouikli, Andromachi UNSPECIFIED orcid.org/0000-0003-1868-6875 UNSPECIFIED Koester, Janis UNSPECIFIED orcid.org/0000-0002-5274-0591 UNSPECIFIED Derisbourg, Maxime J. UNSPECIFIED UNSPECIFIED UNSPECIFIED Chacon-Martinez, Carlos Andres UNSPECIFIED UNSPECIFIED UNSPECIFIED Latza, Christian UNSPECIFIED UNSPECIFIED UNSPECIFIED Antebi, Adam UNSPECIFIED UNSPECIFIED UNSPECIFIED Tessarz, Peter UNSPECIFIED orcid.org/0000-0002-6953-9835 UNSPECIFIED Wickstroem, Sara A. UNSPECIFIED UNSPECIFIED UNSPECIFIED Denzel, Martin S. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-582367
DOI:	10.1242/jcs.252767
Journal or Publication Title:	J. Cell Sci.
Volume:	134
Number:	9
Date:	2021
Publisher:	COMPANY BIOLOGISTS LTD
Place of Publication:	CAMBRIDGE
ISSN:	1477-9137
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language SPERMIDINE-SPERMINE N-1-ACETYLTRANSFERASE; ORNITHINE-DECARBOXYLASE; PROTEIN-SYNTHESIS; OSTEOGENIC DIFFERENTIATION; PROMOTES TRANSLATION; POLYAMINE DEPLETION; SELF-RENEWAL; INHIBITION; CYCLE; IDENTIFICATION Multiple languages Cell Biology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/58236