Universität zu Köln

Oekmen, Bilal, Schwammbach, Daniela, Bakkeren, Guus ORCID: 0000-0002-3065-6989, Neumann, Ulla and Doehlemann, Gunther ORCID: 0000-0002-7353-8456 (2021). The Ustilago hordei-Barley Interaction is a Versatile System for Characterization of Fungal Effectors. J. Fungi, 7 (2). BASEL: MDPI. ISSN 2309-608X

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Abstract

Obligate biotrophic fungal pathogens, such as Blumeria graminis and Puccinia graminis, are amongst the most devastating plant pathogens, causing dramatic yield losses in many economically important crops worldwide. However, a lack of reliable tools for the efficient genetic transformation has hampered studies into the molecular basis of their virulence or pathogenicity. In this study, we present the Ustilago hordei-barley pathosystem as a model to characterize effectors from different plant pathogenic fungi. We generate U. hordei solopathogenic strains, which form infectious filaments without the presence of a compatible mating partner. Solopathogenic strains are suitable for heterologous expression system for fungal virulence factors. A highly efficient Crispr/Cas9 gene editing system is made available for U. hordei. In addition, U. hordei infection structures during barley colonization are analyzed using transmission electron microscopy, showing that U. hordei forms intracellular infection structures sharing high similarity to haustoria formed by obligate rust and powdery mildew fungi. Thus, U. hordei has high potential as a fungal expression platform for functional studies of heterologous effector proteins in barley.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Oekmen, Bilal UNSPECIFIED UNSPECIFIED UNSPECIFIED Schwammbach, Daniela UNSPECIFIED UNSPECIFIED UNSPECIFIED Bakkeren, Guus UNSPECIFIED orcid.org/0000-0002-3065-6989 UNSPECIFIED Neumann, Ulla UNSPECIFIED UNSPECIFIED UNSPECIFIED Doehlemann, Gunther UNSPECIFIED orcid.org/0000-0002-7353-8456 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-583193
DOI:	10.3390/jof7020086
Journal or Publication Title:	J. Fungi
Volume:	7
Number:	2
Date:	2021
Publisher:	MDPI
Place of Publication:	BASEL
ISSN:	2309-608X
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language SMUT FUNGUS; ELECTRON-MICROSCOPY; DISEASE RESISTANCE; SECRETED PROTEINS; HOST; WHEAT; GENE; HAUSTORIA; PATHOGEN; GENOME Multiple languages Microbiology; Mycology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/58319