Universität zu Köln

Reif, Tatjana, Dyckhoff, Gerhard, Hohenberger, Ralph, Kolbe, Carl-Christian, Gruell, Henning, Klein, Florian, Latz, Eicke, Stolp, Bettina and Fackler, Oliver T. (2021). Contact-dependent inhibition of HIV-1 replication in ex vivo human tonsil cultures by polymorphonuclear neutrophils. Cell Rep. Med., 2 (6). AMSTERDAM: ELSEVIER. ISSN 2666-3791

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Abstract

Polymorphonuclear neutrophils (PMNs), the most abundant white blood cells, are recruited rapidly to sites of infection to exert potent anti-microbial activity. Information regarding their role in infection with human immunodeficiency virus (HIV) is limited. Here we report that addition of PMNs to HIV-infected cultures of human tonsil tissue or peripheral blood mononuclear cells causes immediate and long-lasting suppression of HIV-1 spread and virus-induced depletion of CD4 T cells. This inhibition of HIV-1 spread strictly requires PMN contact with infected cells and is not mediated by soluble factors. 2-Photon (2PM) imaging visualized contacts of PMNs with HIV-1-infected CD4 T cells in tonsil tissue that do not result in lysis or uptake of infected cells. The anti-HIV activity of PMNs also does not involve degranulation, formation of neutrophil extracellular traps, or integrin-dependent cell communication. These results reveal that PMNs efficiently blunt HIV-1 replication in primary target cells and tissue by an unconventional mechanism.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Reif, Tatjana UNSPECIFIED UNSPECIFIED UNSPECIFIED Dyckhoff, Gerhard UNSPECIFIED UNSPECIFIED UNSPECIFIED Hohenberger, Ralph UNSPECIFIED UNSPECIFIED UNSPECIFIED Kolbe, Carl-Christian UNSPECIFIED UNSPECIFIED UNSPECIFIED Gruell, Henning UNSPECIFIED UNSPECIFIED UNSPECIFIED Klein, Florian UNSPECIFIED UNSPECIFIED UNSPECIFIED Latz, Eicke UNSPECIFIED UNSPECIFIED UNSPECIFIED Stolp, Bettina UNSPECIFIED UNSPECIFIED UNSPECIFIED Fackler, Oliver T. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-584798
DOI:	10.1016/j.xcrm.2021.100317
Journal or Publication Title:	Cell Rep. Med.
Volume:	2
Number:	6
Date:	2021
Publisher:	ELSEVIER
Place of Publication:	AMSTERDAM
ISSN:	2666-3791
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language IMMUNODEFICIENCY-VIRUS TYPE-1; HERPES-SIMPLEX-VIRUS; EXTRACELLULAR TRAPS; T-CELLS; INFECTION; TRANSMISSION; INNATE; PHAGOCYTOSIS; CYTOTOXICITY; MACROPHAGES Multiple languages Cell Biology; Medicine, Research & Experimental Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/58479