Universität zu Köln

Klimka, Alexander ORCID: 0000-0002-8027-679X, Mertins, Sonja, Nicolai, Anne Kristin, Rummler, Liza Marie, Higgins, Paul G. ORCID: 0000-0001-8677-9454, Gunther, Saskia Diana, Tosetti, Bettina, Krut, Oleg and Kronke, Martin (2021). Epitope-specific immunity against Staphylococcus aureus coproporphyrinogen III oxidase. npj Vaccines, 6 (1). BERLIN: NATURE RESEARCH. ISSN 2059-0105

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Abstract

Staphylococcus aureus represents a serious infectious threat to global public health and a vaccine against S. aureus represents an unmet medical need. We here characterise two S. aureus vaccine candidates, coproporphyrinogen III oxidase (CgoX) and triose phosphate isomerase (TPI), which fulfil essential housekeeping functions in heme synthesis and glycolysis, respectively. Immunisation with rCgoX and rTPI elicited protective immunity against S. aureus bacteremia. Two monoclonal antibodies (mAb), CgoX-D3 and TPI-H8, raised against CgoX and TPI, efficiently provided protection against S. aureus infection. MAb-CgoX-D3 recognised a linear epitope spanning 12 amino acids (aa), whereas TPI-H8 recognised a larger discontinuous epitope. The CgoX-D3 epitope conjugated to BSA elicited a strong, protective immune response against S. aureus infection. The CgoX-D3 epitope is highly conserved in clinical S. aureus isolates, indicating its potential wide usability against S. aureus infection. These data suggest that immunofocusing through epitope-based immunisation constitutes a strategy for the development of a S. aureus vaccine with greater efficacy and better safety profile.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Klimka, Alexander UNSPECIFIED orcid.org/0000-0002-8027-679X UNSPECIFIED Mertins, Sonja UNSPECIFIED UNSPECIFIED UNSPECIFIED Nicolai, Anne Kristin UNSPECIFIED UNSPECIFIED UNSPECIFIED Rummler, Liza Marie UNSPECIFIED UNSPECIFIED UNSPECIFIED Higgins, Paul G. UNSPECIFIED orcid.org/0000-0001-8677-9454 UNSPECIFIED Gunther, Saskia Diana UNSPECIFIED UNSPECIFIED UNSPECIFIED Tosetti, Bettina UNSPECIFIED UNSPECIFIED UNSPECIFIED Krut, Oleg UNSPECIFIED UNSPECIFIED UNSPECIFIED Kronke, Martin UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-585828
DOI:	10.1038/s41541-020-00268-2
Journal or Publication Title:	npj Vaccines
Volume:	6
Number:	1
Date:	2021
Publisher:	NATURE RESEARCH
Place of Publication:	BERLIN
ISSN:	2059-0105
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language TRIOSEPHOSPHATE ISOMERASE; HEREDITARY COPROPORPHYRIA; VACCINE; ANTIBODIES; PROTEIN; IDENTIFICATION; PLASMINOGEN; INFECTIONS Multiple languages Immunology; Medicine, Research & Experimental Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/58582