Universität zu Köln

Sommer, Julian, Gerbracht, Kristina M., Krause, Felix F., Wild, Florian, Tietgen, Manuela, Riedel-Christ, Sara, Sattler, Janko ORCID: 0000-0003-1415-4169, Hamprecht, Axel ORCID: 0000-0003-1449-5780, Kempf, Volkhard A. J. and Goettig, Stephan (2021). OXA-484, an OXA-48-Type Carbapenem-Hydrolyzing Class D beta-Lactamase From Escherichia coli. Front. Microbiol., 12. LAUSANNE: FRONTIERS MEDIA SA. ISSN 1664-302X

Full text not available from this repository.

Abstract

OXA-48-like carbapenemases are among the most frequent carbapenemases in Gram-negative Enterobacterales worldwide with the highest prevalence in the Middle East, North Africa and Europe. Here, we investigated the so far uncharacterized carbapenemase OXA-484 from a clinical E. coli isolate belonging to the high-risk clone ST410 regarding antibiotic resistance pattern, horizontal gene transfer (HGT) and genetic support. OXA-484 differs by the amino acid substitution 214G compared to the most closely related variants OXA-181 (214R) and OXA-232 (214S). The bla(OXA)(-)(484) was carried on a self-transmissible 51.5 kb IncX3 plasmid (pOXA-484) showing high sequence similarity with plasmids harboring bla(OXA)(-)(181). Intraspecies and intergenus HGT of pOXA-484 to different recipients occurred at low frequencies of 1.4 x 10(-7) to 2.1 x 10(-6). OXA-484 increased MICs of temocillin and carbapenems similar to OXA-232 and OXA-244, but lower compared with OXA-48 and OXA-181. Hence, OXA-484 combines properties of OXA-181-like plasmid support and transferability as well as beta-lactamase activity of OXA-232.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Sommer, Julian UNSPECIFIED UNSPECIFIED UNSPECIFIED Gerbracht, Kristina M. UNSPECIFIED UNSPECIFIED UNSPECIFIED Krause, Felix F. UNSPECIFIED UNSPECIFIED UNSPECIFIED Wild, Florian UNSPECIFIED UNSPECIFIED UNSPECIFIED Tietgen, Manuela UNSPECIFIED UNSPECIFIED UNSPECIFIED Riedel-Christ, Sara UNSPECIFIED UNSPECIFIED UNSPECIFIED Sattler, Janko UNSPECIFIED orcid.org/0000-0003-1415-4169 UNSPECIFIED Hamprecht, Axel UNSPECIFIED orcid.org/0000-0003-1449-5780 UNSPECIFIED Kempf, Volkhard A. J. UNSPECIFIED UNSPECIFIED UNSPECIFIED Goettig, Stephan UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-586485
DOI:	10.3389/fmicb.2021.660094
Journal or Publication Title:	Front. Microbiol.
Volume:	12
Date:	2021
Publisher:	FRONTIERS MEDIA SA
Place of Publication:	LAUSANNE
ISSN:	1664-302X
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language SEQUENCE; PLASMID Multiple languages Microbiology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/58648