Universität zu Köln

Lee, Namkyu ORCID: 0000-0002-0342-9764, Afanasenkau, Dzmitry, Rinklin, Philipp ORCID: 0000-0003-1063-8342, Wolfrum, Bernhard ORCID: 0000-0003-4438-3755 and Wiegand, Simone ORCID: 0000-0001-6333-1956 (2021). Temperature profile characterization with fluorescence lifetime imaging microscopy in a thermophoretic chip. Eur. Phys. J. E, 44 (10). NEW YORK: SPRINGER. ISSN 1292-895X

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Abstract

This study introduces a thermophoretic lab-on-a-chip device to measure the Soret coefficient. We use resistive heating of a microwire on the chip to induce a temperature gradient, which is measured by fluorescence lifetime imaging microscopy (FLIM). To verify the functionality of the device, we used dyed polystyrene particles with a diameter of 25 nm. A confocal microscope is utilized to monitor the concentration profile of colloidal particles in the temperature field. Based on the measured temperature and concentration differences, we calculate the corresponding Soret coefficient. The same particles have been recently investigated with thermal diffusion forced Rayleigh scattering (TDFRS) and we find that the obtained Soret coefficients agree with literature results. This chip offers a simple way to study the thermophoretic behavior of biological systems in multicomponent buffer solutions quantitatively, which are difficult to study with optical methods solely relying on the refractive index contrast.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Lee, Namkyu UNSPECIFIED orcid.org/0000-0002-0342-9764 UNSPECIFIED Afanasenkau, Dzmitry UNSPECIFIED UNSPECIFIED UNSPECIFIED Rinklin, Philipp UNSPECIFIED orcid.org/0000-0003-1063-8342 UNSPECIFIED Wolfrum, Bernhard UNSPECIFIED orcid.org/0000-0003-4438-3755 UNSPECIFIED Wiegand, Simone UNSPECIFIED orcid.org/0000-0001-6333-1956 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-587584
DOI:	10.1140/epje/s10189-021-00133-7
Journal or Publication Title:	Eur. Phys. J. E
Volume:	44
Number:	10
Date:	2021
Publisher:	SPRINGER
Place of Publication:	NEW YORK
ISSN:	1292-895X
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language THERMAL-DIFFUSION; SORET; THERMODIFFUSION; COEFFICIENTS; SEPARATION; MIXTURES Multiple languages Chemistry, Physical; Materials Science, Multidisciplinary; Physics, Applied; Polymer Science Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/58758