Universität zu Köln

Frerigmann, Henning, Piotrowski, Markus, Lemke, Rene, Bednarek, Pawel ORCID: 0000-0002-3064-7775 and Schulze-Lefert, Paul (2021). A Network of Phosphate Starvation and Immune-Related Signaling and Metabolic Pathways Controls the Interaction between Arabidopsis thaliana and the Beneficial Fungus Colletotrichum tofieldiae. Mol. Plant-Microbe Interact., 34 (5). S. 560 - 571. ST PAUL: AMER PHYTOPATHOLOGICAL SOC. ISSN 1943-7706

Full text not available from this repository.

Abstract

The beneficial root-colonizing fungus Colletotrichum tofieldiae mediates plant growth promotion (PGP) upon phosphate (Pi) starvation in Arabidopsis thaliana. This activity is dependent on the Trp metabolism of the host, including indole glucosinolate (IG) hydrolysis. Here, we show that C. tofieldiae resolves several Pi starvation-induced molecular processes in the host, one of which is the downregulation of auxin signaling in germ-free plants, which is restored in the presence of the fungus. Using CRISPR/Cas9 genome editing, we generated an Arabidopsis triple mutant lacking three homologous nitrilases (NIT1 to NIT3) that are thought to link IG-hydrolysis products with auxin biosynthesis. Retained C. tofieldiae-induced PGP in nit1/2/3 mutant plants demonstrated that this metabolic connection is dispensable for the beneficial activity of the fungus. This suggests that either there is an alternative metabolic link between IG-hydrolysis products and auxin biosynthesis, or C. tofieldiae restores auxin signaling independently of IG metabolism. We show that C. tofieldiae, similar to pathogenic microorganisms, triggers Arabidopsis immune pathways that rely on IG metabolism as well as salicylic acid and ethylene signaling. Analysis of IG-deficient myb mutants revealed that these metabolites are, indeed, important for control of in planta C. tofieldiae growth: however, enhanced C. tofieldiae biomass does not necessarily negatively correlate with PGP. We show that Pi deficiency enables more efficient colonization of Arabidopsis by C. tofieldiae, possibly due to the MYC2-mediated repression of ethylene signaling and changes in the constitutive IG composition in roots.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Frerigmann, Henning UNSPECIFIED UNSPECIFIED UNSPECIFIED Piotrowski, Markus UNSPECIFIED UNSPECIFIED UNSPECIFIED Lemke, Rene UNSPECIFIED UNSPECIFIED UNSPECIFIED Bednarek, Pawel UNSPECIFIED orcid.org/0000-0002-3064-7775 UNSPECIFIED Schulze-Lefert, Paul UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-587860
DOI:	10.1094/MPMI-08-20-0233-R
Journal or Publication Title:	Mol. Plant-Microbe Interact.
Volume:	34
Number:	5
Page Range:	S. 560 - 571
Date:	2021
Publisher:	AMER PHYTOPATHOLOGICAL SOC
Place of Publication:	ST PAUL
ISSN:	1943-7706
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language AUXIN BIOSYNTHESIS; TRYPTOPHAN-METABOLISM; SECONDARY METABOLITES; NONHOST RESISTANCE; ROOT-SYSTEM; PLANT; JASMONATE; EXPRESSION; MECHANISM; POINT Multiple languages Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Plant Sciences Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/58786