Universität zu Köln

Sun, Mei, Zafrullah, Nabeel ORCID: 0000-0003-2145-1372, Adams, Sheila, Devaux, Floriane, Avila, Marcel Y., Ziebarth, Noel, Margo, Curtis E., Koch, Manuel ORCID: 0000-0002-2962-7814 and Espana, Edgar M. (2021). Collagen XIV Is an Intrinsic Regulator of Corneal Stromal Structure and Function. Am. J. Pathol., 191 (12). S. 2184 - 2195. NEW YORK: ELSEVIER SCIENCE INC. ISSN 1525-2191

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Abstract

Collagen XIV is poorly characterized in the body, and the current knowledge of its function in the cornea is limited. The aim of the current study was to elucidate the role(s) of collagen XIV in regulating corneal stromal structure and function. Analysis of collagen XIV expression, temporal and spatial, was performed at different postnatal days (Ps) in wild-type C57BL/6 mouse corneal stromas and after injury. Conventional collagen XIV null mice were used to inquire the roles that collagen XIV plays in fibrillogenesis, fibril packing, and tissue mechanics. Fibril assembly and packing as well as stromal organization were evaluated using transmission electron microscopy and second harmonic generation microscopy. Atomic force microscopy was used to assess stromal stiffness. Co114a1 mRNA expression was present at P4 to P10 and decreased at P30. No immunoreactivity was noted at P150. Abnormal collagen fibril assembly with a shift toward larger-diameter fibrils and increased interfibrillar spacing in the absence of collagen XIV was found. Second harmonic generation microscopy showed impaired fibrillogenesis in the collagen XIV null stroma. Mechanical testing suggested that collagen XIV confers stiffness to stromal tissue. Expression of collagen XIV is up-regulated following injury. This study indicates that collagen XIV plays a regulatory role in corneal development and in the function of the adult cornea. The expression of collagen XIV is recapitulated during wound healing.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Sun, Mei UNSPECIFIED UNSPECIFIED UNSPECIFIED Zafrullah, Nabeel UNSPECIFIED orcid.org/0000-0003-2145-1372 UNSPECIFIED Adams, Sheila UNSPECIFIED UNSPECIFIED UNSPECIFIED Devaux, Floriane UNSPECIFIED UNSPECIFIED UNSPECIFIED Avila, Marcel Y. UNSPECIFIED UNSPECIFIED UNSPECIFIED Ziebarth, Noel UNSPECIFIED UNSPECIFIED UNSPECIFIED Margo, Curtis E. UNSPECIFIED UNSPECIFIED UNSPECIFIED Koch, Manuel UNSPECIFIED orcid.org/0000-0002-2962-7814 UNSPECIFIED Espana, Edgar M. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-588484
DOI:	10.1016/j.ajpath.2021.08.016
Journal or Publication Title:	Am. J. Pathol.
Volume:	191
Number:	12
Page Range:	S. 2184 - 2195
Date:	2021
Publisher:	ELSEVIER SCIENCE INC
Place of Publication:	NEW YORK
ISSN:	1525-2191
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language EXPRESSION; FIBRILLOGENESIS; SKIN; BINDING; PROTEIN; GROWTH; ROLES Multiple languages Pathology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/58848