Universität zu Köln

Brych, Annika, Haas, Fabian B., Parzefall, Katharina, Panzer, Sabine, Schermuly, Jeanette, Altmueller, Janine, Engelsdorf, Timo ORCID: 0000-0002-2169-0536, Terpitz, Ulrich ORCID: 0000-0003-3031-3422, Rensing, Stefan A., Kiontke, Stephan and Batschauer, Alfred (2021). Coregulation of gene expression by White collar 1 and phytochrome in Ustilago maydis. Fungal Genet. Biol., 152. SAN DIEGO: ACADEMIC PRESS INC ELSEVIER SCIENCE. ISSN 1096-0937

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Abstract

Ustilago maydis encodes ten predicted light-sensing proteins. The biological functions of only a few of them are elucidated. Among the characterized ones are two DNA-photolyases and two rhodopsins that act as DNA-repair enzymes or green light-driven proton pumps, respectively. Here we report on the role of two other photoreceptors in U. maydis, namely White collar 1 (Wco1) and Phytochrome 1 (Phy1). We show that they bind flavins or biliverdin as chromophores, respectively. Both photoreceptors undergo a photocycle in vitro. Wco1 is the dominant blue light receptor in the saprophytic phase, controlling all of the 324 differentially expressed genes in blue light. U. maydis also responds to red and far-red light. However, the number of red or far-red light-controlled genes is less compared to blue light-regulated ones. Moreover, most of the red and far-red light-controlled genes not only depend on Phy1 but also on Wco1, indicating partial coregulation of gene expression by both photoreceptors. GFP-fused Wco1 is preferentially located in the nucleus, Phy1 in the cytosol, thus providing no hint that these photoreceptors directly interact or operate within the same complex. This is the first report on a functional characterization and coaction of White collar 1 and phytochrome orthologs in basidiomycetes.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Brych, Annika UNSPECIFIED UNSPECIFIED UNSPECIFIED Haas, Fabian B. UNSPECIFIED UNSPECIFIED UNSPECIFIED Parzefall, Katharina UNSPECIFIED UNSPECIFIED UNSPECIFIED Panzer, Sabine UNSPECIFIED UNSPECIFIED UNSPECIFIED Schermuly, Jeanette UNSPECIFIED UNSPECIFIED UNSPECIFIED Altmueller, Janine UNSPECIFIED UNSPECIFIED UNSPECIFIED Engelsdorf, Timo UNSPECIFIED orcid.org/0000-0002-2169-0536 UNSPECIFIED Terpitz, Ulrich UNSPECIFIED orcid.org/0000-0003-3031-3422 UNSPECIFIED Rensing, Stefan A. UNSPECIFIED UNSPECIFIED UNSPECIFIED Kiontke, Stephan UNSPECIFIED UNSPECIFIED UNSPECIFIED Batschauer, Alfred UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-588796
DOI:	10.1016/j.fgb.2021.103570
Journal or Publication Title:	Fungal Genet. Biol.
Volume:	152
Date:	2021
Publisher:	ACADEMIC PRESS INC ELSEVIER SCIENCE
Place of Publication:	SAN DIEGO
ISSN:	1096-0937
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language BLUE-LIGHT PHOTORECEPTOR; ASPERGILLUS-NIDULANS; RNA-SEQ; DIFFERENTIAL EXPRESSION; PATHOGENIC DEVELOPMENT; SIGNAL-TRANSDUCTION; REPLACEMENT MUTANTS; REGULATED GENES; DNA-REPAIR; NEUROSPORA Multiple languages Genetics & Heredity; Mycology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/58879