Data from a microdosed recombinant human erythropoietin administration study applying the new biotinylated clone AE7A5 antibody and a further optimized sarcosyl polyacrylamide gel electrophoresis protocol

Reichel, Christian, Erceg, Damir, Lorenc, Barbara, Scheiblhofer, Veronika, Farmer, Letizia, Zanitzer, Katharina, Geisendorfer, Thomas, Gmeiner, Gunter and Thevis, Mario . Data from a microdosed recombinant human erythropoietin administration study applying the new biotinylated clone AE7A5 antibody and a further optimized sarcosyl polyacrylamide gel electrophoresis protocol. Drug Test. Anal.. HOBOKEN: WILEY. ISSN 1942-7611

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DOI:10.1002/dta.2989

Abstract

Erythropoietin (EPO) is a hormone, which stimulates the production of red blood cells. Due to its performance-enhancing effect, it is prohibited by the World Anti-Doping Agency (WADA). In order to reduce the detection window of EPO doping, athletes have been applying low doses of recombinant EPO (e.g., <10 IU/kg body weight, daily or every second day) instead of larger doses twice or more per week (e.g., 30 IU/kg). Microdoses of Retacrit (epoetin zeta), an EPO biosimilar, were administered intravenously and subcutaneously to human males and females. Urine and serum samples were collected and analysed applying the new biotinylated clone AE7A5 EPO antibody and a further optimized sarcosyl polyacrylamide gel electrophoresis (SAR-PAGE) protocol. With the improved protocol, microdosed Retacrit (7.5 IU/kg body weight [BW]) was detectable for at least 52 h after intravenous administration. Detection windows were approximately the same for serum and urine and doubled after subcutaneous administration (similar to 10(4) h). Previous studies applying different electrophoretic techniques and the not further optimized SAR-PAGE protocol revealed considerably shorter detection windows for recombinant human erythropoietin (rhEPO) microdoses. Because the new biotinylated antibody performed significantly more sensitive than the nonbiotinylated version, the new protocol will improve the sensitivity and hence detectability of recombinant EPO in doping control.

Item Type:

Journal Article

Creators:

Creators	Email	ORCID	ORCID Put Code
Reichel, Christian	UNSPECIFIED	UNSPECIFIED	UNSPECIFIED
Erceg, Damir	UNSPECIFIED	UNSPECIFIED	UNSPECIFIED
Lorenc, Barbara	UNSPECIFIED	UNSPECIFIED	UNSPECIFIED
Scheiblhofer, Veronika	UNSPECIFIED	UNSPECIFIED	UNSPECIFIED
Farmer, Letizia	UNSPECIFIED	UNSPECIFIED	UNSPECIFIED
Zanitzer, Katharina	UNSPECIFIED	UNSPECIFIED	UNSPECIFIED
Geisendorfer, Thomas	UNSPECIFIED	UNSPECIFIED	UNSPECIFIED
Gmeiner, Gunter	UNSPECIFIED	UNSPECIFIED	UNSPECIFIED
Thevis, Mario	UNSPECIFIED	UNSPECIFIED	UNSPECIFIED