Universität zu Köln

Behrens, Maik ORCID: 0000-0003-2082-8860, Di Pizio, Antonella, Redel, Ulrike, Meyerhof, Wolfgang and Korsching, Sigrun, I (2021). At the Root of T2R Gene Evolution: Recognition Profiles of Coelacanth and Zebrafish Bitter Receptors. Genome Biol. Evol., 13 (1). OXFORD: OXFORD UNIV PRESS. ISSN 1759-6653

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Abstract

The careful evaluation of food is important for survival throughout the animal kingdom, and specialized chemoreceptors have evolved to recognize nutrients, minerals, acids, andmany toxins. Vertebrate bitter taste, mediated by the taste receptor type 2 (T2R) family, warns against potentially toxic compounds. During evolution T2R receptors appear first in bony fish, but the functional properties of bony fish T2R receptors are mostly unknown. We performed a phylogenetic analysis showing the living fossil coelacanth (Latimeria chalumnae) and zebrafish (Danio rerio) to possess T2R repertoires typical for early-diverged species in the lobe-finned and the ray-finned clade, respectively. Receptors from these two species were selected for heterologous expression assays using a diverse panel of bitter substances. Remarkably, the ligand profile of the most basal coelacanth receptor, T2R01, is identical to that of its ortholog in zebrafish, consistent with functional conservation across >400 Myr of separate evolution. The second coelacanth receptor deorphaned, T2R02, is activated by steroid hormones and bile acids, evolutionary oldmolecules that are potentially endogenously synthesized agonists for extraoral T2Rs. For zebrafish, we report the presence of both specialized and promiscuous T2R receptors. Moreover, we identified an antagonist for one of the zebrafish receptors suggesting that bitter antagonism contributed to shape this receptor family throughout evolution.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Behrens, Maik UNSPECIFIED orcid.org/0000-0003-2082-8860 UNSPECIFIED Di Pizio, Antonella UNSPECIFIED UNSPECIFIED UNSPECIFIED Redel, Ulrike UNSPECIFIED UNSPECIFIED UNSPECIFIED Meyerhof, Wolfgang UNSPECIFIED UNSPECIFIED UNSPECIFIED Korsching, Sigrun, I UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-589026
DOI:	10.1093/gbe/evaa264
Journal or Publication Title:	Genome Biol. Evol.
Volume:	13
Number:	1
Date:	2021
Publisher:	OXFORD UNIV PRESS
Place of Publication:	OXFORD
ISSN:	1759-6653
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language TASTE RECEPTOR; LATIMERIA-CHALUMNAE; PHYLOGENETIC ANALYSIS; FAMILY; FISH; SEQUENCE; SEARCH; REQUIREMENTS; SACCHARIN; SELECTION Multiple languages Evolutionary Biology; Genetics & Heredity Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/58902