Universität zu Köln

Winkels, Holger, Ghosheh, Yanal, Kobiyama, Kouji, Kiosses, William B., Orecchioni, Marco, Ehinger, Erik, Suryawanshi, Vasantika ORCID: 0000-0003-1497-7480, Herrera-De la Mata, Sara, Marchovecchio, Paola, Riffelmacher, Thomas ORCID: 0000-0002-0372-5272, Thiault, Nicolas, Kronenberg, Mitchell, Wolf, Dennis ORCID: 0000-0003-1525-4348, Seumois, Gregory, Vijayanand, Pandurangan and Ley, Klaus (2021). Thymus-Derived CD4(+)CD8(+) Cells Reside in Mediastinal Adipose Tissue and the Aortic Arch. J. Immunol., 207 (11). S. 2720 - 2734. BETHESDA: AMER ASSOC IMMUNOLOGISTS. ISSN 1550-6606

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Abstract

Double-positive CD4(+)CD8 alpha beta(+) (DP) cells are thought to reside as T cell progenitors exclusively within the thymus. We recently discovered an unexpected CD4(+) and CD8 alpha beta(+)immune cell population in healthy and atherosclerotic mice by single-cell RNA sequencing. Transcriptomically, these cells resembled thymic DPs. Flow cytometry and three-dimensional whole-mount imaging confirmed DPs in thymus, mediastinal adipose tissue, and aortic adventitia, but nowhere else. Deep transcriptional profiling revealed differences between DP cells isolated from the three locations. All DPs were dependent on RAG2 expression and the presence of the thymus. Mediastinal adipose tissue DPs resided in close vicinity to invariant NKT cells, which they could activate in vitro. Thymus transplantation failed to reconstitute extrathymic DPs, and frequencies of extrathymic DPs were unaltered by pharmacologic inhibition of S1P1, suggesting that their migration may be locally confined. Our results define two new, transcriptionally distinct subsets of extrathymic DPs that may play a role in aortic vascular homeostasis.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Winkels, Holger UNSPECIFIED UNSPECIFIED UNSPECIFIED Ghosheh, Yanal UNSPECIFIED UNSPECIFIED UNSPECIFIED Kobiyama, Kouji UNSPECIFIED UNSPECIFIED UNSPECIFIED Kiosses, William B. UNSPECIFIED UNSPECIFIED UNSPECIFIED Orecchioni, Marco UNSPECIFIED UNSPECIFIED UNSPECIFIED Ehinger, Erik UNSPECIFIED UNSPECIFIED UNSPECIFIED Suryawanshi, Vasantika UNSPECIFIED orcid.org/0000-0003-1497-7480 UNSPECIFIED Herrera-De la Mata, Sara UNSPECIFIED UNSPECIFIED UNSPECIFIED Marchovecchio, Paola UNSPECIFIED UNSPECIFIED UNSPECIFIED Riffelmacher, Thomas UNSPECIFIED orcid.org/0000-0002-0372-5272 UNSPECIFIED Thiault, Nicolas UNSPECIFIED UNSPECIFIED UNSPECIFIED Kronenberg, Mitchell UNSPECIFIED UNSPECIFIED UNSPECIFIED Wolf, Dennis UNSPECIFIED orcid.org/0000-0003-1525-4348 UNSPECIFIED Seumois, Gregory UNSPECIFIED UNSPECIFIED UNSPECIFIED Vijayanand, Pandurangan UNSPECIFIED UNSPECIFIED UNSPECIFIED Ley, Klaus UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-589043
DOI:	10.4049/jimmunol.2100208
Journal or Publication Title:	J. Immunol.
Volume:	207
Number:	11
Page Range:	S. 2720 - 2734
Date:	2021
Publisher:	AMER ASSOC IMMUNOLOGISTS
Place of Publication:	BETHESDA
ISSN:	1550-6606
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language CERVICAL THYMUS; T-CELLS; PRE-TCR; RNA; EXPRESSION; SELECTION; ATHEROSCLEROSIS; PROGRANULIN; REPERTOIRE; MATURATION Multiple languages Immunology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/58904