Zhang, Shu, Uebelmesser, Nadine, Josipovic, Natasa ORCID: 0000-0001-7683-0869, Forte, Giada ORCID: 0000-0001-9939-4465, Slotman, Johan A. ORCID: 0000-0001-9705-9620, Chiang, Michael ORCID: 0000-0002-1620-2550, Gothe, Henrike, Gusmao, Eduardo Gade, Becker, Christian, Altmueller, Janine, Houtsmuller, Adriaan B., Roukos, Vassilis, Wendt, Kerstin S., Marenduzzo, Davide and Papantonis, Argyris (2021). RNA polymerase II is required for spatial chromatin reorganization following exit from mitosis. Sci. Adv., 7 (43). WASHINGTON: AMER ASSOC ADVANCEMENT SCIENCE. ISSN 2375-2548

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Abstract

Mammalian chromosomes are three-dimensional entities shaped by converging and opposing forces. Mitotic cell division induces marked chromosome condensation, but following reentry into the G(1) phase of the cell cycle, chromosomes reestablish their interphase organization. Here, we tested the role of RNA polymerase II (RNAPII) in this transition using a cell line that allows its auxin-mediated degradation. In situ Hi-C showed that RNAPII is required for both compartment and loop establishment following mitosis. RNAPs often counteract loop extrusion, and in their absence, longer and more prominent loops arose. Evidence from chromatin binding, super-resolution imaging, and in silico modeling allude to these effects being a result of RNAPII-mediated cohesin loading upon G(1) reentry. Our findings reconcile the role of RNAPII in gene expression with that in chromatin architecture.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Zhang, ShuUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Uebelmesser, NadineUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Josipovic, NatasaUNSPECIFIEDorcid.org/0000-0001-7683-0869UNSPECIFIED
Forte, GiadaUNSPECIFIEDorcid.org/0000-0001-9939-4465UNSPECIFIED
Slotman, Johan A.UNSPECIFIEDorcid.org/0000-0001-9705-9620UNSPECIFIED
Chiang, MichaelUNSPECIFIEDorcid.org/0000-0002-1620-2550UNSPECIFIED
Gothe, HenrikeUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Gusmao, Eduardo GadeUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Becker, ChristianUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Altmueller, JanineUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Houtsmuller, Adriaan B.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Roukos, VassilisUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Wendt, Kerstin S.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Marenduzzo, DavideUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Papantonis, ArgyrisUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
URN: urn:nbn:de:hbz:38-591267
DOI: 10.1126/sciadv.abg8205
Journal or Publication Title: Sci. Adv.
Volume: 7
Number: 43
Date: 2021
Publisher: AMER ASSOC ADVANCEMENT SCIENCE
Place of Publication: WASHINGTON
ISSN: 2375-2548
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
HUMAN COHESIN; X-CHROMOSOME; TRANSCRIPTION; ORGANIZATION; DOMAINS; LOOPS; CTCF; DNA; DEGRADATION; ACTIVATIONMultiple languages
Multidisciplinary SciencesMultiple languages
URI: http://kups.ub.uni-koeln.de/id/eprint/59126

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