Universität zu Köln

Curien, Gilles, Lyska, Dagmar ORCID: 0000-0002-9175-6334, Guglielmino, Erika, Westhoff, Phillip, Janetzko, Janina, Tardif, Marianne, Hallopeau, Clement ORCID: 0000-0002-0692-2407, Brugiere, Sabine, Dal Bo, Davide, Decelle, Johan ORCID: 0000-0002-4343-8358, Gallet, Benoit, Falconet, Denis, Carone, Michele ORCID: 0000-0002-5561-0792, Remacle, Claire ORCID: 0000-0002-5016-9547, Ferro, Myriam, Weber, Andreas P. M. and Finazzi, Giovanni (2021). Mixotrophic growth of the extremophile Galdieria sulphuraria reveals the flexibility of its carbon assimilation metabolism. New Phytol., 231 (1). S. 326 - 339. HOBOKEN: WILEY. ISSN 1469-8137

Full text not available from this repository.

Abstract

Galdieria sulphuraria is a cosmopolitan microalga found in volcanic hot springs and calderas. It grows at low pH in photoautotrophic (use of light as a source of energy) or heterotrophic (respiration as a source of energy) conditions, using an unusually broad range of organic carbon sources. Previous data suggested that G. sulphuraria cannot grow mixotrophically (simultaneously exploiting light and organic carbon as energy sources), its photosynthetic machinery being repressed by organic carbon. Here, we show that G. sulphuraria SAG21.92 thrives in photoautotrophy, heterotrophy and mixotrophy. By comparing growth, biomass production, photosynthetic and respiratory performances in these three trophic modes, we show that addition of organic carbon to cultures (mixotrophy) relieves inorganic carbon limitation of photosynthesis thanks to increased CO2 supply through respiration. This synergistic effect is lost when inorganic carbon limitation is artificially overcome by saturating photosynthesis with added external CO2. Proteomic and metabolic profiling corroborates this conclusion suggesting that mixotrophy is an opportunistic mechanism to increase intracellular CO2 concentration under physiological conditions, boosting photosynthesis by enhancing the carboxylation activity of Ribulose-1,5-bisphosphate carboxylase-oxygenase (Rubisco) and decreasing photorespiration. We discuss possible implications of these findings for the ecological success of Galdieria in extreme environments and for biotechnological applications.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Curien, Gilles UNSPECIFIED UNSPECIFIED UNSPECIFIED Lyska, Dagmar UNSPECIFIED orcid.org/0000-0002-9175-6334 UNSPECIFIED Guglielmino, Erika UNSPECIFIED UNSPECIFIED UNSPECIFIED Westhoff, Phillip UNSPECIFIED UNSPECIFIED UNSPECIFIED Janetzko, Janina UNSPECIFIED UNSPECIFIED UNSPECIFIED Tardif, Marianne UNSPECIFIED UNSPECIFIED UNSPECIFIED Hallopeau, Clement UNSPECIFIED orcid.org/0000-0002-0692-2407 UNSPECIFIED Brugiere, Sabine UNSPECIFIED UNSPECIFIED UNSPECIFIED Dal Bo, Davide UNSPECIFIED UNSPECIFIED UNSPECIFIED Decelle, Johan UNSPECIFIED orcid.org/0000-0002-4343-8358 UNSPECIFIED Gallet, Benoit UNSPECIFIED UNSPECIFIED UNSPECIFIED Falconet, Denis UNSPECIFIED UNSPECIFIED UNSPECIFIED Carone, Michele UNSPECIFIED orcid.org/0000-0002-5561-0792 UNSPECIFIED Remacle, Claire UNSPECIFIED orcid.org/0000-0002-5016-9547 UNSPECIFIED Ferro, Myriam UNSPECIFIED UNSPECIFIED UNSPECIFIED Weber, Andreas P. M. UNSPECIFIED UNSPECIFIED UNSPECIFIED Finazzi, Giovanni UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-593894
DOI:	10.1111/nph.17359
Journal or Publication Title:	New Phytol.
Volume:	231
Number:	1
Page Range:	S. 326 - 339
Date:	2021
Publisher:	WILEY
Place of Publication:	HOBOKEN
ISSN:	1469-8137
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language RIBULOSE 1,5-BISPHOSPHATE CARBOXYLASE; ALGA CYANIDIOSCHYZON MEROLAE; THERMOPHILIC RED ALGA; ORGANIC-CARBON; INORGANIC CARBON; MOLECULAR-BASIS; ELECTRON FLOW; PHOTOSYNTHESIS; LIGHT; CO2 Multiple languages Plant Sciences Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/59389