Universität zu Köln

Nemec, Alexandr ORCID: 0000-0003-0608-9598, Radolfova-Krizova, Lenka, Maixnerova, Martina, Nemec, Matej, Spanelova, Petra, Safrankova, Renata, Sedo, Ondrej, Lopes, Bruno S. and Higgins, Paul G. ORCID: 0000-0001-8677-9454 (2021). Delineation of a novel environmental phylogroup of the genus Acinetobacter encompassing Acinetobacter terrae sp. nov., Acinetobacter terrestris sp. nov. and three other tentative species. Syst. Appl. Microbiol., 44 (4). MUNICH: ELSEVIER GMBH. ISSN 1618-0984

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Abstract

This study aimed to define the taxonomic position and structure of a novel, taxonomically unique group of 26 Acinetobacter strains, provisionally designated Taxon 24 (T24). The strains were recovered from soil and freshwater ecosystems (n = 21) or animals (n = 5) in Czechia, Scotland, Germany, the Netherlands and Turkey between 1993 and 2015. They were non-glucose-acidifying, nonhemolytic, nonproteolytic, growing at 32 degrees C and on acetate and ethanol as single carbon sources, but not on 4-hydroxybenzoate and mostly not at 37 degrees C. Their whole-genome sequences were 3.0-3.7 Mb in size, with GC contents of 39.8-41.3%. Based on core genome phylogenetic analysis, the 26 strains formed a distinct Glade within the genus Acinetobacter, with strongly supported subclades termed T24A (n = 11), T24B (n = 8), T24C (n = 2), T24D (n = 3) and T24E (n = 2). The internal genomic ANIb values for these subclades were >94.8%, while the ANIb values between them were <92.5%. The results of MALDI-TOF MS-based analyses agreed with this classification. The five subclades differed from each other in the results of one to six carbon source assimilation tests. Given the genomic and phenotypic distinctness, internal coherence, numbers of available strains and geographically diverse origin of T24A and T24B, we propose the names Acinetobacter terrae sp. nov. and Acinetobacter terrestris sp. nov. for these two taxa, respectively. The type strains are ANC 4282(v) (= CCM 8986(T) = CCUG 73811(T) = CNCTC 8082(T)) and ANC 4471(T) (= CCM 8985(T) = CCUG 73812(T) = CNCTC 8093(T)), respectively. We conclude that these two species together with the other T24 strains represent a widely dispersed Acinetobacter Glade primarily associated with terrestrial ecosystems. (C) 2021 Elsevier GmbH. All rights reserved.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Nemec, Alexandr UNSPECIFIED orcid.org/0000-0003-0608-9598 UNSPECIFIED Radolfova-Krizova, Lenka UNSPECIFIED UNSPECIFIED UNSPECIFIED Maixnerova, Martina UNSPECIFIED UNSPECIFIED UNSPECIFIED Nemec, Matej UNSPECIFIED UNSPECIFIED UNSPECIFIED Spanelova, Petra UNSPECIFIED UNSPECIFIED UNSPECIFIED Safrankova, Renata UNSPECIFIED UNSPECIFIED UNSPECIFIED Sedo, Ondrej UNSPECIFIED UNSPECIFIED UNSPECIFIED Lopes, Bruno S. UNSPECIFIED UNSPECIFIED UNSPECIFIED Higgins, Paul G. UNSPECIFIED orcid.org/0000-0001-8677-9454 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-593960
DOI:	10.1016/j.syapm.2021.126217
Journal or Publication Title:	Syst. Appl. Microbiol.
Volume:	44
Number:	4
Date:	2021
Publisher:	ELSEVIER GMBH
Place of Publication:	MUNICH
ISSN:	1618-0984
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language NATURAL SOIL Multiple languages Biotechnology & Applied Microbiology; Microbiology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/59396