Universität zu Köln

Biagi Jr, Carlos Alberto Oliveira, Cury, Sarah Santiloni, Alves, Cleidson Padua, Rabhi, Nabil, Silva Jr, Wilson Araujo ORCID: 0000-0001-9364-2886, Farmer, Stephen R., Carvalho, Robson Francisco ORCID: 0000-0002-4901-7714 and Batista Jr, Miguel Luiz (2021). Multidimensional Single-Nuclei RNA-Seq Reconstruction of Adipose Tissue Reveals Adipocyte Plasticity Underlying Thermogenic Response. Cells, 10 (11). BASEL: MDPI. ISSN 2073-4409

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Abstract

Adipose tissue has been classified based on its morphology and function as white, brown, or beige/brite. It plays an essential role as a regulator of systemic metabolism through paracrine and endocrine signals. Recently, multiple adipocyte subtypes have been revealed using RNA sequencing technology, going beyond simply defined morphology but also by their cellular origin, adaptation to metabolic stress, and plasticity. Here, we performed an in-depth analysis of publicly available single-nuclei RNAseq from adipose tissue and utilized a workflow template to characterize adipocyte plasticity, heterogeneity, and secretome profiles. The reanalyzed dataset led to the identification of different subtypes of adipocytes including three subpopulations of thermogenic adipocytes, and provided a characterization of distinct transcriptional profiles along the adipocyte trajectory under thermogenic challenges. This study provides a useful resource for further investigations regarding mechanisms related to adipocyte plasticity and trans-differentiation.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Biagi Jr, Carlos Alberto Oliveira UNSPECIFIED UNSPECIFIED UNSPECIFIED Cury, Sarah Santiloni UNSPECIFIED UNSPECIFIED UNSPECIFIED Alves, Cleidson Padua UNSPECIFIED UNSPECIFIED UNSPECIFIED Rabhi, Nabil UNSPECIFIED UNSPECIFIED UNSPECIFIED Silva Jr, Wilson Araujo UNSPECIFIED orcid.org/0000-0001-9364-2886 UNSPECIFIED Farmer, Stephen R. UNSPECIFIED UNSPECIFIED UNSPECIFIED Carvalho, Robson Francisco UNSPECIFIED orcid.org/0000-0002-4901-7714 UNSPECIFIED Batista Jr, Miguel Luiz UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-594257
DOI:	10.3390/cells10113073
Journal or Publication Title:	Cells
Volume:	10
Number:	11
Date:	2021
Publisher:	MDPI
Place of Publication:	BASEL
ISSN:	2073-4409
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language TRANSCRIPTIONAL CONTROL; BROWN; CELL; WHITE; BEIGE; IDENTIFICATION; ENVIRONMENT; EXPRESSION; CYTOSCAPE; MOUSE Multiple languages Cell Biology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/59425