Universität zu Köln

Bock, Theresa ORCID: 0000-0002-8308-4021, Turk, Clara, Aravamudhan, Sriram, Keufgens, Lena, Bloch, Wilhelm, Rozsivalova, Dieu Hien, Romanello, Vanina, Nogara, Leonardo, Blaauw, Bert, Trifunovic, Aleksandra ORCID: 0000-0002-5472-3517, Braun, Thomas and Krueger, Marcus (2021). PERM1 interacts with the MICOS-MIB complex to connect the mitochondria and sarcolemma via ankyrin B. Nat. Commun., 12 (1). BERLIN: NATURE PORTFOLIO. ISSN 2041-1723

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Abstract

Skeletal muscle subsarcolemmal mitochondria (SSM) and intermyofibrillar mitochondria subpopulations have distinct metabolic activity and sensitivity, though the mechanisms that localize SSM to peripheral areas of muscle fibers are poorly understood. A protein interaction study and complexome profiling identifies PERM1 interacts with the MICOS-MIB complex. Ablation of Perm1 in mice reduces muscle force, decreases mitochondrial membrane potential and complex I activity, and reduces the numbers of SSM in skeletal muscle. We demonstrate PERM1 interacts with the intracellular adaptor protein ankyrin B (ANKB) that connects the cytoskeleton to the plasma membrane. Moreover, we identify a C-terminal transmembrane helix that anchors PERM1 into the outer mitochondrial membrane. We conclude PERM1 functions in the MICOS-MIB complex and acts as an adapter to connect the mitochondria with the sarcolemma via ANKB. Mitochondria in skeletal muscle have distinct localization and properties through unclear mechanisms. Here, the authors use complexome profiling and immunoprecipitations to identify PERM1 as a MICOS-MIB complex interactor that also binds ankyrin B, suggesting PERM1 directs the mitochondria to the membrane.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Bock, Theresa UNSPECIFIED orcid.org/0000-0002-8308-4021 UNSPECIFIED Turk, Clara UNSPECIFIED UNSPECIFIED UNSPECIFIED Aravamudhan, Sriram UNSPECIFIED UNSPECIFIED UNSPECIFIED Keufgens, Lena UNSPECIFIED UNSPECIFIED UNSPECIFIED Bloch, Wilhelm UNSPECIFIED UNSPECIFIED UNSPECIFIED Rozsivalova, Dieu Hien UNSPECIFIED UNSPECIFIED UNSPECIFIED Romanello, Vanina UNSPECIFIED UNSPECIFIED UNSPECIFIED Nogara, Leonardo UNSPECIFIED UNSPECIFIED UNSPECIFIED Blaauw, Bert UNSPECIFIED UNSPECIFIED UNSPECIFIED Trifunovic, Aleksandra UNSPECIFIED orcid.org/0000-0002-5472-3517 UNSPECIFIED Braun, Thomas UNSPECIFIED UNSPECIFIED UNSPECIFIED Krueger, Marcus UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-594642
DOI:	10.1038/s41467-021-25185-3
Journal or Publication Title:	Nat. Commun.
Volume:	12
Number:	1
Date:	2021
Publisher:	NATURE PORTFOLIO
Place of Publication:	BERLIN
ISSN:	2041-1723
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language STRUCTURAL ORGANIZATION; INTERMEDIATE-FILAMENTS; OXIDATIVE CAPACITY; CONTACT SITE; PROTEIN; BIOGENESIS; EXERCISE; METABOLISM; RESISTANCE; SOFTWARE Multiple languages Multidisciplinary Sciences Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/59464