Universität zu Köln

Timm, Stefan ORCID: 0000-0003-3105-6296, Nunes-Nesi, Adriano ORCID: 0000-0002-9581-9355, Florian, Alexandra, Eisenhut, Marion ORCID: 0000-0002-2743-8630, Morgenthal, Katja, Wirtz, Markus ORCID: 0000-0001-7790-4022, Hell, Rudiger, Weckwerth, Wolfram, Hagemann, Martin, Fernie, Alisdair R. and Bauwe, Hermann (2021). Metabolite Profiling in Arabidopsis thaliana with Moderately Impaired Photorespiration Reveals Novel Metabolic Links and Compensatory Mechanisms of Photorespiration. Metabolites, 11 (6). BASEL: MDPI. ISSN 2218-1989

Full text not available from this repository.

Abstract

Photorespiration is an integral component of plant primary metabolism. Accordingly, it has been often observed that impairing the photorespiratory flux negatively impacts other cellular processes. In this study, the metabolic acclimation of the Arabidopsis thaliana wild type was compared with the hydroxypyruvate reductase 1 (HPR1; hpr1) mutant, displaying only a moderately reduced photorespiratory flux. Plants were analyzed during development and under varying photoperiods with a combination of non-targeted and targeted metabolome analysis, as well as C-13- and C-14-labeling approaches. The results showed that HPR1 deficiency is more critical for photorespiration during the vegetative compared to the regenerative growth phase. A shorter photoperiod seems to slowdown the photorespiratory metabolite conversion mostly at the glycerate kinase and glycine decarboxylase steps compared to long days. It is demonstrated that even a moderate impairment of photorespiration severely reduces the leaf-carbohydrate status and impacts on sulfur metabolism. Isotope labeling approaches revealed an increased CO2 release from hpr1 leaves, most likely occurring from enhanced non-enzymatic 3-hydroxypyruvate decarboxylation and a higher flux from serine towards ethanolamine through serine decarboxylase. Collectively, the study provides evidence that the moderate hpr1 mutant is an excellent tool to unravel the underlying mechanisms governing the regulation of metabolic linkages of photorespiration with plant primary metabolism.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Timm, Stefan UNSPECIFIED orcid.org/0000-0003-3105-6296 UNSPECIFIED Nunes-Nesi, Adriano UNSPECIFIED orcid.org/0000-0002-9581-9355 UNSPECIFIED Florian, Alexandra UNSPECIFIED UNSPECIFIED UNSPECIFIED Eisenhut, Marion UNSPECIFIED orcid.org/0000-0002-2743-8630 UNSPECIFIED Morgenthal, Katja UNSPECIFIED UNSPECIFIED UNSPECIFIED Wirtz, Markus UNSPECIFIED orcid.org/0000-0001-7790-4022 UNSPECIFIED Hell, Rudiger UNSPECIFIED UNSPECIFIED UNSPECIFIED Weckwerth, Wolfram UNSPECIFIED UNSPECIFIED UNSPECIFIED Hagemann, Martin UNSPECIFIED UNSPECIFIED UNSPECIFIED Fernie, Alisdair R. UNSPECIFIED UNSPECIFIED UNSPECIFIED Bauwe, Hermann UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-594750
DOI:	10.3390/metabo11060391
Journal or Publication Title:	Metabolites
Volume:	11
Number:	6
Date:	2021
Publisher:	MDPI
Place of Publication:	BASEL
ISSN:	2218-1989
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language GLYCINE DECARBOXYLASE; NITRATE ASSIMILATION; GENE-EXPRESSION; CARBON-DIOXIDE; SERINE; PHOTOSYNTHESIS; GLYCERATE; PLANTS; STRESS; GROWTH Multiple languages Biochemistry & Molecular Biology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/59475