Universität zu Köln

Wagner, Isabel Viola, Kloeting, Nora, Savchuk, Luliia, Eifler, Lisa, Kulle, Alexandra, Kralisch-Jaecklein, Susan, Doetsch, Jorg, Hiort, Olaf, Svechnikov, Konstantin and Soder, Olle (2021). Diabetes Type 1 Negatively Influences Leydig Cell Function in Rats, Which is Partially Reversible By Insulin Treatment. Endocrinology, 162 (4). WASHINGTON: ENDOCRINE SOC. ISSN 1945-7170

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Abstract

Type 1 diabetes mellitus (T1DM) is associated with impaired spermatogenesis and lower testosterone levels and epididymal weight. However, the underlying processes in the testis are unknown and remain to be elucidated. Therefore, the present study focused on the effects ofT1DM on testicular function in a spontaneously diabetic rat model. BB/OKL rats after diabetes manifestation were divided into 3 groups: those without insulin treatment and insulin treatment for a duration of 2 and of 6 weeks. Anthropometrical data, circulating levels of gonadotrophins, testosterone, and inhibin B were measured. Intratesticular testosterone, oxidative stress, inflammation, and apoptosis were analyzed. Key enzymes of steroidogenesis were evaluated in the testis. Untreated diabetic rats had significantly lower serum follicle-stimulating hormone and luteinizing hormone levels. Serum and intratesticular testosterone levels significantly decreased in untreated diabetic rats compared to healthy controls. Key markers of Leydig cell function were significantly downregulated at the RNA level: insulin-like factor 3 (Ins13) by 53% (P= .006), Star by 51% (P= .004), Cyp11A1 by 80% (P= .003), 3Beta-Hsd2 by 61% (P= .005), and Pbr by 52% (P= .002). In the insulin-treated group, only Cyp11A1 and 3Beta-Hsd2 transcripts were significantly lower. Interestingly, the long-term insulin-treated group showed significant upregulation of most steroidogenic enzymes without affecting testosterone levels. Tumor necrosis factor a and apoptosis were significantly increased in the long-term insulin-treated rats. In conclusion T1DM, with a severe lack of insulin, has an adverse action on Leydig cell function.This is partially reversible with well-compensated blood glucose control. Long-term T1DM adversely affects Leydig cell function because of the process of inflammation and apoptosis.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Wagner, Isabel Viola UNSPECIFIED UNSPECIFIED UNSPECIFIED Kloeting, Nora UNSPECIFIED UNSPECIFIED UNSPECIFIED Savchuk, Luliia UNSPECIFIED UNSPECIFIED UNSPECIFIED Eifler, Lisa UNSPECIFIED UNSPECIFIED UNSPECIFIED Kulle, Alexandra UNSPECIFIED UNSPECIFIED UNSPECIFIED Kralisch-Jaecklein, Susan UNSPECIFIED UNSPECIFIED UNSPECIFIED Doetsch, Jorg UNSPECIFIED UNSPECIFIED UNSPECIFIED Hiort, Olaf UNSPECIFIED UNSPECIFIED UNSPECIFIED Svechnikov, Konstantin UNSPECIFIED UNSPECIFIED UNSPECIFIED Soder, Olle UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-594772
DOI:	10.1210/endocr/bqab017
Journal or Publication Title:	Endocrinology
Volume:	162
Number:	4
Date:	2021
Publisher:	ENDOCRINE SOC
Place of Publication:	WASHINGTON
ISSN:	1945-7170
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language GLYCATION END-PRODUCTS; REPRODUCTIVE-TRACT; ULTRASTRUCTURAL-CHANGES; INDUCED HYPERGLYCEMIA; SEXUAL-BEHAVIOR; SERTOLI-CELL; MELLITUS; PITUITARY; TESTES; MEN Multiple languages Endocrinology & Metabolism Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/59477