Phagocytosis by stroma confounds coculture studies - Kölner UniversitätsPublikationsServer

Herbst, Sophie A., Stolarczyk, Marta, Becirovic, Tina, Czernilofsky, Felix, Liu, Yi, Kolb, Carolin, Knoll, Mareike, Herling, Marco, Mueller-Tidow, Carsten and Dietrich, Sascha (2021). Phagocytosis by stroma confounds coculture studies. iScience, 24 (9). CAMBRIDGE: CELL PRESS. ISSN 2589-0042

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DOI:10.1016/j.isci.2021.103062

Abstract

Signals provided by the microenvironment can modify and circumvent pathway activities that are therapeutically targeted by drugs. Bone marrow stromal cell coculture models are frequently used to study the influence of the bone marrow niche on ex vivo drug response. Here, we show that mesenchymal stromal cells from selected donors and NKTert, a stromal cell line, which is commonly used for coculture studies with primary leukemia cells, extensively phagocytose apoptotic cells. This could lead to misinterpretation of results, especially if viability readouts of the target cells (e.g. leukemic cells) in such coculture models are based on the relative proportions of dead and alive cells. Future coculture studies which aim to investigate the impact of bone marrow stromal cells on drug response should take into account that stromal cells have the capacity to phagocytose apoptotic cells.

Item Type:

Journal Article

Creators:

Creators	Email	ORCID	ORCID Put Code
Herbst, Sophie A.	UNSPECIFIED	UNSPECIFIED	UNSPECIFIED
Stolarczyk, Marta	UNSPECIFIED	UNSPECIFIED	UNSPECIFIED
Becirovic, Tina	UNSPECIFIED	UNSPECIFIED	UNSPECIFIED
Czernilofsky, Felix	UNSPECIFIED	UNSPECIFIED	UNSPECIFIED
Liu, Yi	UNSPECIFIED	UNSPECIFIED	UNSPECIFIED
Kolb, Carolin	UNSPECIFIED	UNSPECIFIED	UNSPECIFIED
Knoll, Mareike	UNSPECIFIED	UNSPECIFIED	UNSPECIFIED
Herling, Marco	UNSPECIFIED	UNSPECIFIED	UNSPECIFIED
Mueller-Tidow, Carsten	UNSPECIFIED	UNSPECIFIED	UNSPECIFIED
Dietrich, Sascha	UNSPECIFIED	UNSPECIFIED	UNSPECIFIED