Universität zu Köln

Tso, Michael K., Turgeon, Paul, Bosche, Bert, Lee, Charles K., Nie, Tian ORCID: 0000-0001-7426-0356, D'Abbondanza, Josephine, Ai, Jinglu, Marsden, Philip A. and Macdonald, R. Loch (2021). Gene expression profiling of brain endothelial cells after experimental subarachnoid haemorrhage. Sci Rep, 11 (1). BERLIN: NATURE RESEARCH. ISSN 2045-2322

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Abstract

Subarachnoid haemorrhage (SAH) is a type of hemorrhagic stroke that is associated with high morbidity and mortality. New effective treatments are needed to improve outcomes. The pathophysiology of SAH is complex and includes early brain injury and delayed cerebral ischemia, both of which are characterized by blood-brain barrier (BBB) impairment. We isolated brain endothelial cells (BECs) from mice subjected to SAH by injection of blood into the prechiasmatic cistern. We used gene expression profiling to identify 707 unique genes (2.8% of transcripts, 403 upregulated, 304 downregulated, 24,865 interrogated probe sets) that were significantly differentially expressed in mouse BECs after SAH. The pathway involving prostaglandin synthesis and regulation was significantly upregulated after SAH, including increased expression of the Ptgs2 gene and its corresponding COX-2 protein. Celecoxib, a selective COX-2 inhibitor, limited upregulation of Ptgs2 in BECs. In this study, we have defined the gene expression profiling of BECs after experimental SAH and provide further insight into BBB pathophysiology, which may be relevant to other neurological diseases such as traumatic brain injury, brain tumours, ischaemic stroke, multiple sclerosis, and neurodegenerative disorders.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Tso, Michael K. UNSPECIFIED UNSPECIFIED UNSPECIFIED Turgeon, Paul UNSPECIFIED UNSPECIFIED UNSPECIFIED Bosche, Bert UNSPECIFIED UNSPECIFIED UNSPECIFIED Lee, Charles K. UNSPECIFIED UNSPECIFIED UNSPECIFIED Nie, Tian UNSPECIFIED orcid.org/0000-0001-7426-0356 UNSPECIFIED D'Abbondanza, Josephine UNSPECIFIED UNSPECIFIED UNSPECIFIED Ai, Jinglu UNSPECIFIED UNSPECIFIED UNSPECIFIED Marsden, Philip A. UNSPECIFIED UNSPECIFIED UNSPECIFIED Macdonald, R. Loch UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-597164
DOI:	10.1038/s41598-021-87301-z
Journal or Publication Title:	Sci Rep
Volume:	11
Number:	1
Date:	2021
Publisher:	NATURE RESEARCH
Place of Publication:	BERLIN
ISSN:	2045-2322
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language BASILAR ARTERY Multiple languages Multidisciplinary Sciences Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/59716