Universität zu Köln

Durr, Julius, Reyt, Guilhem ORCID: 0000-0003-0545-2500, Spaepen, Stijn ORCID: 0000-0001-5465-8287, Hilton, Sally, Meehan, Cathal, Qi, Wu, Kamiya, Takehiro ORCID: 0000-0003-3790-7119, Flis, Paulina, Dickinson, Hugh G., Feher, Attila, Shivshankar, Umashankar, Pavagadhi, Shruti, Swarup, Sanjay, Salt, David, Bending, Gary D. and Gutierrez-Marcos, Jose (2021). A Novel Signaling Pathway Required for Arabidopsis Endodermal Root Organization Shapes the Rhizosphere Microbiome. Plant Cell Physiol., 62 (2). S. 248 - 262. OXFORD: OXFORD UNIV PRESS. ISSN 1471-9053

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Abstract

The Casparian strip (CS) constitutes a physical diffusion barrier to water and nutrients in plant roots, which is formed by the polar deposition of lignin polymer in the endodermis tissue. The precise pattern of lignin deposition is determined by the scaffolding activity of membrane-bound Casparian Strip domain proteins (CASPs), but little is known of the mechanism (s) directing this process. Here, we demonstrate that Endodermis-specific Receptor-like Kinase 1 (ERK1) and, to a lesser extent, ROP Binding Kinasel (RBK1) are also involved in regulating CS formation, with the former playing an essential role in lignin deposition as well as in the localization of CASP1. We show that ERK1 is localized to the cytoplasm and nucleus of the endodermis and that together with the circadian clock regulator, Time for Coffee (TIC), forms part of a novel signaling pathway necessary for correct CS organization and suberization of the endodermis, with their single or combined loss of function resulting in altered root microbiome composition. In addition, we found that other mutants displaying defects in suberin deposition at the CS also display altered root exudates and microbiome composition. Thus, our work reveals a complex network of signaling factors operating within the root endodermis that establish both the CS diffusion barrier and influence the microbial composition of the rhizosphere.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Durr, Julius UNSPECIFIED UNSPECIFIED UNSPECIFIED Reyt, Guilhem UNSPECIFIED orcid.org/0000-0003-0545-2500 UNSPECIFIED Spaepen, Stijn UNSPECIFIED orcid.org/0000-0001-5465-8287 UNSPECIFIED Hilton, Sally UNSPECIFIED UNSPECIFIED UNSPECIFIED Meehan, Cathal UNSPECIFIED UNSPECIFIED UNSPECIFIED Qi, Wu UNSPECIFIED UNSPECIFIED UNSPECIFIED Kamiya, Takehiro UNSPECIFIED orcid.org/0000-0003-3790-7119 UNSPECIFIED Flis, Paulina UNSPECIFIED UNSPECIFIED UNSPECIFIED Dickinson, Hugh G. UNSPECIFIED UNSPECIFIED UNSPECIFIED Feher, Attila UNSPECIFIED UNSPECIFIED UNSPECIFIED Shivshankar, Umashankar UNSPECIFIED UNSPECIFIED UNSPECIFIED Pavagadhi, Shruti UNSPECIFIED UNSPECIFIED UNSPECIFIED Swarup, Sanjay UNSPECIFIED UNSPECIFIED UNSPECIFIED Salt, David UNSPECIFIED UNSPECIFIED UNSPECIFIED Bending, Gary D. UNSPECIFIED UNSPECIFIED UNSPECIFIED Gutierrez-Marcos, Jose UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-598876
DOI:	10.1093/pcp/pcaa170
Journal or Publication Title:	Plant Cell Physiol.
Volume:	62
Number:	2
Page Range:	S. 248 - 262
Date:	2021
Publisher:	OXFORD UNIV PRESS
Place of Publication:	OXFORD
ISSN:	1471-9053
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language STRIP DIFFUSION BARRIER; CASPARIAN STRIP; PARTIAL RESISTANCE; CIRCADIAN CLOCK; POWDERY MILDEW; SUBERIN; DEPOSITION; TRANSPORT; BINDING; GATEKEEPERS Multiple languages Plant Sciences; Cell Biology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/59887