Universität zu Köln

Tanida, Konstantin, Hahn, Andreas ORCID: 0000-0002-5157-8369, Eberhardt, Kirsten Alexandra, Tannich, Egbert ORCID: 0000-0002-4714-7275, Landt, Olfert, Kann, Simone, Feldt, Torsten, Sarfo, Fred Stephen, Di Cristanziano, Veronica, Frickmann, Hagen and Loderstaedt, Ulrike (2021). Comparative Assessment of In-House Real-Time PCRs Targeting Enteric Disease-Associated Microsporidia in Human Stool Samples. Pathogens, 10 (6). BASEL: MDPI. ISSN 2076-0817

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Abstract

Microsporidiosis is an infection predominantly occurring in immunosuppressed patients and infrequently also in travelers. This study was performed to comparatively evaluate the diagnostic accuracy of real-time PCR assays targeting microsporidia with etiological relevance in the stool of human patients in a latent class analysis-based test comparison without a reference standard with perfect accuracy. Thereby, two one-tube real-time PCR assays and two two-tube real-time PCR assays targeting Enterocytozoon bieneusi and Encephalocytozoon spp. were included in the assessment with reference stool material (20), stool samples from Ghanaian HIV-positive patients (903), and from travelers, migrants and Colombian indigenous people (416). Sensitivity of the assays ranged from 60.4% to 97.4% and specificity from 99.1% to 100% with substantial agreement according to Cohen's kappa of 79.6%. Microsporidia DNA was detected in the reference material and the stool of the HIV patients but not in the stool of the travelers, migrants, and the Colombian indigenous people. Accuracy-adjusted prevalence was 5.8% (n = 78) for the study population as a whole. In conclusion, reliable detection of enteric disease-associated microsporidia in stool samples by real-time PCR could be demonstrated, but sensitivity between the compared microsporidia-specific real-time PCR assays varied.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Tanida, Konstantin UNSPECIFIED UNSPECIFIED UNSPECIFIED Hahn, Andreas UNSPECIFIED orcid.org/0000-0002-5157-8369 UNSPECIFIED Eberhardt, Kirsten Alexandra UNSPECIFIED UNSPECIFIED UNSPECIFIED Tannich, Egbert UNSPECIFIED orcid.org/0000-0002-4714-7275 UNSPECIFIED Landt, Olfert UNSPECIFIED UNSPECIFIED UNSPECIFIED Kann, Simone UNSPECIFIED UNSPECIFIED UNSPECIFIED Feldt, Torsten UNSPECIFIED UNSPECIFIED UNSPECIFIED Sarfo, Fred Stephen UNSPECIFIED UNSPECIFIED UNSPECIFIED Di Cristanziano, Veronica UNSPECIFIED UNSPECIFIED UNSPECIFIED Frickmann, Hagen UNSPECIFIED UNSPECIFIED UNSPECIFIED Loderstaedt, Ulrike UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-599841
DOI:	10.3390/pathogens10060656
Journal or Publication Title:	Pathogens
Volume:	10
Number:	6
Date:	2021
Publisher:	MDPI
Place of Publication:	BASEL
ISSN:	2076-0817
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language ENTEROCYTOZOON-BIENEUSI; QUANTITATIVE DETECTION; PREVALENCE; INFECTION; SPECIMENS; PATHOGENS; ASSAY Multiple languages Microbiology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/59984