Jurmeister, Philipp, Vollbrecht, Claudia ORCID: 0000-0002-0861-001X, Joehrens, Korinna, Aust, Daniela, Behnke, Anke, Stenzinger, Albrecht, Penzel, Roland, Endris, Volker, Schirmacher, Peter, Fisseler-Eckhoff, Annette, Neumann, Jens, Kirchner, Thomas, Buettner, Reinhard, Merkelbach-Bruse, Sabine, Kreipe, Hans, Jonigk, Danny, Jochum, Wolfram, Rodriguez, Regulo, Dietel, Manfred, Horst, David, Hummel, Michael ORCID: 0000-0001-6717-605X and von Laffert, Maximilian (2021). Status quo of ALK testing in lung cancer: results of an EQA scheme based on in-situ hybridization, immunohistochemistry, and RNA/DNA sequencing. Virchows Arch., 479 (2). S. 247 - 256. NEW YORK: SPRINGER. ISSN 1432-2307

Full text not available from this repository.

Abstract

With this external quality assessment (EQA) scheme, we aim to investigate the diagnostic performance of the currently available methods for the detection of ALK alterations in non-small cell lung cancer on a national scale, namely, in situ hybridization (ISH), immunohistochemistry (IHC), and RNA/DNA sequencing (NGS). The EQA scheme cohort consisted of ten specimens, including four ALK positive and six ALK negative samples, which were thoroughly pretested using IHC, ISH, and RNA/DNA NGS. Unstained tumor sections were provided to the 57 participants, and the results were retrieved via an online questionnaire. ISH was used by 29, IHC by 38, and RNA/DNA sequencing by 19 participants. Twenty-eight institutions (97%) passed the ring trial using ISH, 33 (87%) by using IHC, and 18 (95%) by using NGS. The highest sensitivity and interrater agreement (Fleiss ' kappa) was observed for RNA/DNA sequencing (99%, 0.975), followed by ISH (94%, 0.898) and IHC (92%, 0.888). However, the proportion of samples that were not evaluable due to bad tissue quality was also higher for RNA/DNA sequencing (4%) compared with ISH (0.7%) and IHC (0.5%). While all three methods produced reliable results between the different institutions, the highest sensitivity and concordance were observed for RNA/DNA sequencing. These findings encourage the broad implementation of this method in routine diagnostic, although the application might be limited by technical capacity, economical restrictions, and tissue quality of formalin-fixed samples.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Jurmeister, PhilippUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Vollbrecht, ClaudiaUNSPECIFIEDorcid.org/0000-0002-0861-001XUNSPECIFIED
Joehrens, KorinnaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Aust, DanielaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Behnke, AnkeUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Stenzinger, AlbrechtUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Penzel, RolandUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Endris, VolkerUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Schirmacher, PeterUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Fisseler-Eckhoff, AnnetteUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Neumann, JensUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Kirchner, ThomasUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Buettner, ReinhardUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Merkelbach-Bruse, SabineUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Kreipe, HansUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Jonigk, DannyUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Jochum, WolframUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Rodriguez, ReguloUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Dietel, ManfredUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Horst, DavidUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Hummel, MichaelUNSPECIFIEDorcid.org/0000-0001-6717-605XUNSPECIFIED
von Laffert, MaximilianUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
URN: urn:nbn:de:hbz:38-602242
DOI: 10.1007/s00428-021-03106-5
Journal or Publication Title: Virchows Arch.
Volume: 479
Number: 2
Page Range: S. 247 - 256
Date: 2021
Publisher: SPRINGER
Place of Publication: NEW YORK
ISSN: 1432-2307
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
ANAPLASTIC LYMPHOMA KINASE; EML4-ALK FUSION; GENE REARRANGEMENT; THORACIC ONCOLOGY; ADENOCARCINOMAS; DIAGNOSTICS; CRIZOTINIB; SURVIVAL; NSCLC; IHCMultiple languages
PathologyMultiple languages
URI: http://kups.ub.uni-koeln.de/id/eprint/60224

Downloads

Downloads per month over past year

Altmetric

Export

Actions (login required)

View Item View Item