Mesny, Fantin ORCID: 0000-0002-3044-1398, Miyauchi, Shingo, Thiergart, Thorsten, Pickel, Brigitte, Atanasova, Lea ORCID: 0000-0002-1751-277X, Karlsson, Magnus ORCID: 0000-0001-6098-138X, Huettel, Bruno, Barry, Kerrie W., Haridas, Sajeet ORCID: 0000-0002-0229-0975, Chen, Cindy, Bauer, Diane, Andreopoulos, William, Pangilinan, Jasmyn, LaButti, Kurt, Riley, Robert ORCID: 0000-0003-0224-0975, Lipzen, Anna, Clum, Alicia, Drula, Elodie, Henrissat, Bernard ORCID: 0000-0002-3434-8588, Kohler, Annegret, Grigoriev, Igor, V, Martin, Francis M. and Hacquard, Stephane (2021). Genetic determinants of endophytism in the Arabidopsis root mycobiome. Nat. Commun., 12 (1). BERLIN: NATURE PORTFOLIO. ISSN 2041-1723

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Abstract

Plant roots host diverse fungal communities that affect plant health. Here, Mesny et al. use comparative genomics and transcriptomics of fungal isolates from the Arabidopsis thaliana root mycobiota, together with root colonization assays, to identify genetic determinants of endophytism. The roots of Arabidopsis thaliana host diverse fungal communities that affect plant health and disease states. Here, we sequence the genomes of 41 fungal isolates representative of the A. thaliana root mycobiota for comparative analysis with other 79 plant-associated fungi. Our analyses indicate that root mycobiota members evolved from ancestors with diverse lifestyles and retain large repertoires of plant cell wall-degrading enzymes (PCWDEs) and effector-like small secreted proteins. We identify a set of 84 gene families associated with endophytism, including genes encoding PCWDEs acting on xylan (family GH10) and cellulose (family AA9). Transcripts encoding these enzymes are also part of a conserved transcriptional program activated by phylogenetically-distant mycobiota members upon host contact. Recolonization experiments with individual fungi indicate that strains with detrimental effects in mono-association with the host colonize roots more aggressively than those with beneficial activities, and dominate in natural root samples. Furthermore, we show that the pectin-degrading enzyme family PL1_7 links aggressiveness of endophytic colonization to plant health.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Mesny, FantinUNSPECIFIEDorcid.org/0000-0002-3044-1398UNSPECIFIED
Miyauchi, ShingoUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Thiergart, ThorstenUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Pickel, BrigitteUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Atanasova, LeaUNSPECIFIEDorcid.org/0000-0002-1751-277XUNSPECIFIED
Karlsson, MagnusUNSPECIFIEDorcid.org/0000-0001-6098-138XUNSPECIFIED
Huettel, BrunoUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Barry, Kerrie W.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Haridas, SajeetUNSPECIFIEDorcid.org/0000-0002-0229-0975UNSPECIFIED
Chen, CindyUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Bauer, DianeUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Andreopoulos, WilliamUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Pangilinan, JasmynUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
LaButti, KurtUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Riley, RobertUNSPECIFIEDorcid.org/0000-0003-0224-0975UNSPECIFIED
Lipzen, AnnaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Clum, AliciaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Drula, ElodieUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Henrissat, BernardUNSPECIFIEDorcid.org/0000-0002-3434-8588UNSPECIFIED
Kohler, AnnegretUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Grigoriev, Igor, VUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Martin, Francis M.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Hacquard, StephaneUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
URN: urn:nbn:de:hbz:38-602337
DOI: 10.1038/s41467-021-27479-y
Journal or Publication Title: Nat. Commun.
Volume: 12
Number: 1
Date: 2021
Publisher: NATURE PORTFOLIO
Place of Publication: BERLIN
ISSN: 2041-1723
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
PLANT; FUNGI; TIME; MECHANISMS; MICROBIOTA; PATHOGENS; THALIANA; DATABASE; ENZYMES; TOOLMultiple languages
Multidisciplinary SciencesMultiple languages
URI: http://kups.ub.uni-koeln.de/id/eprint/60233

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