Universität zu Köln

Esser, Ruth L., Brueck, Carolin, Thiele, Jan, Golumba-Nagy, Viktoria, Meyer, Anja ORCID: 0000-0001-9904-5070, Steinbach-Knoedgen, Eva, Yan, Shuaifeng, Pesch, Carola Tho, Stahl, David, Schiller, Joanna and Kofler, David M. (2022). Truncated isoforms of GPSM2 containing the GoLoco motif region promote CD4(+) T-cell migration in SLE. Lupus Sci. Med., 9 (1). LONDON: BMJ PUBLISHING GROUP. ISSN 2053-8790

Full text not available from this repository.

Abstract

Objective SLE is an autoimmune disease with a complex pathogenesis. T-cell infiltration into organs contributes to inflammation and organ damage in SLE. Recently, G-protein signalling modulator 2 (GPSM2) has been shown to be implicated in T-cell migration. Methods We analysed the expression levels of GPSM2 and of a truncated isoform of GPSM2 containing the GoLoco motif region in CD4(+) T cells from patients with SLE and from healthy individuals by western blot. In a next step, we studied the role of the truncated GPSM2 isoform using a CD4(+) T-cell migration assay. Results Our experiments revealed comparable levels of GPSM2 in CD4(+) T cells from patients with SLE and healthy controls. In contrast, the truncated 35 kDa isoform of GPSM2 was significantly more highly expressed in CD4(+) T cells from patients with SLE as compared with healthy subjects. Antibody-mediated blockade of the 35 kDa GPSM2 isoform reduced the in vitro capacity of CD4(+) T cells to migrate towards the chemokine CCL20. Conclusions A truncated GPSM2 isoform containing the GoLoco motif region is upregulated in CD4(+) T cells from patients with SLE and promotes CD4(+) T-cell migration. Targeting this isoform with specific antibodies might be a promising approach to reduce CD4(+) T-cell infiltration into inflamed tissues and to prevent organ damage in SLE.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Esser, Ruth L. UNSPECIFIED UNSPECIFIED UNSPECIFIED Brueck, Carolin UNSPECIFIED UNSPECIFIED UNSPECIFIED Thiele, Jan UNSPECIFIED UNSPECIFIED UNSPECIFIED Golumba-Nagy, Viktoria UNSPECIFIED UNSPECIFIED UNSPECIFIED Meyer, Anja UNSPECIFIED orcid.org/0000-0001-9904-5070 UNSPECIFIED Steinbach-Knoedgen, Eva UNSPECIFIED UNSPECIFIED UNSPECIFIED Yan, Shuaifeng UNSPECIFIED UNSPECIFIED UNSPECIFIED Pesch, Carola Tho UNSPECIFIED UNSPECIFIED UNSPECIFIED Stahl, David UNSPECIFIED UNSPECIFIED UNSPECIFIED Schiller, Joanna UNSPECIFIED UNSPECIFIED UNSPECIFIED Kofler, David M. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-658695
DOI:	10.1136/lupus-2022-000742
Journal or Publication Title:	Lupus Sci. Med.
Volume:	9
Number:	1
Date:	2022
Publisher:	BMJ PUBLISHING GROUP
Place of Publication:	LONDON
ISSN:	2053-8790
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language EXPRESSION Multiple languages Rheumatology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/65869