Universität zu Köln

Gloyer, L., Golumba-Nagy, V, Meyer, A., Yan, S., Schiller, J., Breuninger, M., Jochimsen, D. and Kofler, D. M. (2022). Adenosine receptor A2a blockade by caffeine increases IFN-gamma production in Th1 cells from patients with rheumatoid arthritis. Scand. J. Rheumatol., 51 (4). S. 279 - 284. ABINGDON: TAYLOR & FRANCIS LTD. ISSN 1502-7732

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Abstract

Objective Studies indicate that caffeine uptake may be a risk factor for rheumatoid arthritis (RA), but a definitive link between caffeine consumption and RA has not been established. This study aimed to investigate the interplay between caffeine, adenosine receptor A2a, and interferon-gamma (IFN-gamma) production in CD4(+) T cells from RA patients. Method Peripheral blood mononuclear cells were obtained from the peripheral blood of healthy individuals and patients with RA. CD4(+) T cells were isolated using the magnetic activated cell sorting technique and cultured in vitro with caffeine or mock control. In addition, adenosine was used as a competitive inhibitor of caffeine. After 48 h, expression of IFN-gamma and interleukin-17 (IL-17) was analysed by flow cytometry. Ex vivo expression levels of adenosine receptor A2a were also assessed. Results Caffeine promoted IFN-gamma production in Th1 cells in vitro. Significantly higher concentrations of caffeine were required to increase IFN-gamma levels in Th1 cells from healthy individuals compared to Th1 cells from patients with RA. Moreover, ex vivo levels of adenosine receptor A2a expression on CD4(+) T cells were significantly higher in RA than in healthy individuals. Caffeine-driven IFN-gamma production was completely reversed by adenosine, a competitive agonist of adenosine receptor A2a. In contrast to IFN-gamma, production of IL-17 was not affected by caffeine. Conclusion Caffeine promotes IFN-gamma production in Th1 cells from RA patients in vitro by competitive inhibition of adenosine receptor A2a. Excessive coffee consumption could contribute to T-cell activation and inflammation in RA.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Gloyer, L. UNSPECIFIED UNSPECIFIED UNSPECIFIED Golumba-Nagy, V UNSPECIFIED UNSPECIFIED UNSPECIFIED Meyer, A. UNSPECIFIED UNSPECIFIED UNSPECIFIED Yan, S. UNSPECIFIED UNSPECIFIED UNSPECIFIED Schiller, J. UNSPECIFIED UNSPECIFIED UNSPECIFIED Breuninger, M. UNSPECIFIED UNSPECIFIED UNSPECIFIED Jochimsen, D. UNSPECIFIED UNSPECIFIED UNSPECIFIED Kofler, D. M. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-663643
DOI:	10.1080/03009742.2021.1995956
Journal or Publication Title:	Scand. J. Rheumatol.
Volume:	51
Number:	4
Page Range:	S. 279 - 284
Date:	2022
Publisher:	TAYLOR & FRANCIS LTD
Place of Publication:	ABINGDON
ISSN:	1502-7732
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language COFFEE CONSUMPTION; RISK Multiple languages Rheumatology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/66364