Universität zu Köln

Piper, Thomas, Fusshoeller, Gregor, Schaenzer, Wilhelm and Thevis, Mario (2022). Investigations on the in vivo metabolism of 5 alpha-androst-2-en-17-one. Rapid Commun. Mass Spectrom., 36 (17). HOBOKEN: WILEY. ISSN 1097-0231

Full text not available from this repository.

Abstract

Rationale The anabolic steroid 5 alpha-androst-2-en-17-one (2EN) is sold as a prohormone and has been investigated regarding its potential as a steroidal aromatase inhibitor. The administration of 2EN was detected in a doping control sample in 2015, and investigations into its metabolism allowed for the identification and characterization of three urinary metabolites. Unfortunately, the utility of the main metabolite 2 beta,3 alpha-dihydroxy-5 alpha-androstan-17-one for doping control purposes was hampered under routine doping control conditions due to chromatographic issues, thus warranting further studies on the metabolism of the prohibited substance. Methods The metabolism of 2EN was reinvestigated after oral administration of twofold-deuterated 2EN employing hydrogen isotope ratio mass spectrometry (IRMS) in combination with high-accuracy/high-resolution mass spectrometry. After a single dose of 50 mg of doubly labeled 2EN, urine samples were collected for 9 days. All samples were processed using routine doping control methods for IRMS analysis, and all detected metabolites were further characterized by mass spectrometry-based investigations. Results More than 15 different metabolites still containing the deuterium label were detected after administration. The presence of steroids exhibiting a 5 beta-configuration was unexpected as the administered 2EN features a 5 alpha-configured pharmacophore. Further investigations corroborated a significant impact of the administered 2EN on etiocholanolone and 5 beta-androstanediol. Seven metabolites of 2EN not present as endogenous compounds were identified as potential candidates for routine doping controls and could be detected for up to 9 days after administration. Conclusions The new metabolites identified in this study enable the detection of the misuse of 2EN for up to 9 days. The conversion of a 5 alpha-steroid to urinary metabolites with 5 beta-configuration has not been reported so far and should be further investigated.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Piper, Thomas UNSPECIFIED UNSPECIFIED UNSPECIFIED Fusshoeller, Gregor UNSPECIFIED UNSPECIFIED UNSPECIFIED Schaenzer, Wilhelm UNSPECIFIED UNSPECIFIED UNSPECIFIED Thevis, Mario UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-663921
DOI:	10.1002/rcm.9343
Journal or Publication Title:	Rapid Commun. Mass Spectrom.
Volume:	36
Number:	17
Date:	2022
Publisher:	WILEY
Place of Publication:	HOBOKEN
ISSN:	1097-0231
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language ENDOGENOUS URINARY STEROIDS; RATIO MASS-SPECTROMETRY; CARBON-ISOTOPE RATIO; REFERENCE-POPULATION; GAS-CHROMATOGRAPHY; C-13/C-12 RATIOS; IDENTIFICATION; TESTOSTERONE Multiple languages Biochemical Research Methods; Chemistry, Analytical; Spectroscopy Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/66392