Universität zu Köln

Goody, Philip Roger, Nachtsheim, Lisa, Hosen, Mohammed Rabiul, von Krosigk, Miriam, Christmann, Dominik, Klussmann, Jens Peter, Zietzer, Andreas, Breitrueck, Nils, Jansen, Felix and Jansen, Stefanie (2022). Analysis of nocturnal, hypoxia-induced miRNAs in sleep apnea patients. PLoS One, 17 (3). SAN FRANCISCO: PUBLIC LIBRARY SCIENCE. ISSN 1932-6203

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Abstract

Introduction Obstructive sleep apnea syndrome (OSAS) is associated with an increased cardiovascular risk. The underlying mechanisms are largely unclear. MicroRNAs (miRNAs) are RNAs circulating in the blood that can be released into the bloodstream during hypoxia. In the present study, we investigate if OSAS-induced hypoxia results in a release of miRNAs that may mediate OSAS-associated cardiovascular damage. Methods Blood was sampled from 23 OSAS patients before and after a polygraphically monitored night. Total circulating RNA was isolated from the plasma and quantified using real-time qPCR. Using a Taqman miRNA array, the levels of 384 different miRNAs were compared between evening and morning after polysomnography. The most highly upregulated miRNA (miRNA-505) and four additionally upregulated miRNAs (miRNA-127, miRNA-133a, miRNA-145, and miRNA-181a) were then quantified in a bigger patient cohort individually. Results Apnea/Hypopnea-Index (AHI) was evaluated and averaged at 26 per hour on nocturnal polygraphy. In an initial miRNA array, a total of 4 miRNAs were significantly regulated. A significant increase of miRNA-145 was observed in the larger patient cohort. No significant changes in concentration were detected for miRNA-127, miRNA-133a, miRNA-181a, and miRNA-505 in this larger cohort. Conclusion OSAS results in the nocturnal release of miRNAs into the bloodstream. Our collected data may indicate a hypoxia-induced release of miRNAs into the bloodstream of OSAS-patients. In vitro experiments are needed to confirm the secretion of these miRNAs under hypoxia and evaluate the effect on the cardio vasculature.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Goody, Philip Roger UNSPECIFIED UNSPECIFIED UNSPECIFIED Nachtsheim, Lisa UNSPECIFIED UNSPECIFIED UNSPECIFIED Hosen, Mohammed Rabiul UNSPECIFIED UNSPECIFIED UNSPECIFIED von Krosigk, Miriam UNSPECIFIED UNSPECIFIED UNSPECIFIED Christmann, Dominik UNSPECIFIED UNSPECIFIED UNSPECIFIED Klussmann, Jens Peter UNSPECIFIED UNSPECIFIED UNSPECIFIED Zietzer, Andreas UNSPECIFIED UNSPECIFIED UNSPECIFIED Breitrueck, Nils UNSPECIFIED UNSPECIFIED UNSPECIFIED Jansen, Felix UNSPECIFIED UNSPECIFIED UNSPECIFIED Jansen, Stefanie UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-666295
DOI:	10.1371/journal.pone.0263747
Journal or Publication Title:	PLoS One
Volume:	17
Number:	3
Date:	2022
Publisher:	PUBLIC LIBRARY SCIENCE
Place of Publication:	SAN FRANCISCO
ISSN:	1932-6203
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language VASCULAR SMOOTH-MUSCLE; PERIPHERAL-BLOOD; EXPRESSION; MICRORNAS; CELLS; ANGIOGENESIS; HYPERTENSION; APOPTOSIS; SIGNATURE; MIR-145 Multiple languages Multidisciplinary Sciences Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/66629