Universität zu Köln

Mattern, Annabelle, Habermann, Sebastian, Zegke, Markus, Wickleder, Mathias Siegfried and Alberto, Roger (2022). High-Yield 99mTc Labeling of Gold Nanoparticles Carrying Atropine and Adrenaline. Bioconjugate Chem., 33 (9). S. 1741 - 1750. WASHINGTON: AMER CHEMICAL SOC. ISSN 1520-4812

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Abstract

This work focuses on the synthesis, purification, and analytical characterization of novel multifunctional Au NPs radiolabeled with 99mTc. These mixed-ligand shell Au NPs represent pharmacologically relevant samples for potential application in theragnostics. A ligand using a plain linker with a rather long chain consisting of 10 CH2 groups and a thiol moiety along with the PADA chelator has been used for both the attachment to the Au NP surface and for the99mTc(CO)3+ complexation. We have combined this with our approach of stabilizing Au NP without any PEG or other stabilizing groups. Thus, monoligand shell Au NPs were radiolabeled by different strategies (prelabeling and postlabeling). Additionally, pharmacologically relevant Au NPs were synthesized carrying both a biofunctionalization with either atropine or adrenaline and the 99mTc radiolabel. All samples were obtained in very good yields (up to 80% of the total activity loaded onto the column) and completely/particularly purified using desalting columns. Detailed analytical characterization of the Au NPs before and after radiolabeling has proven the NPs' robustness throughout the process. Their intact functionalization, shape, and stability was confirmed by transmission electron microscopy (TEM), ultraviolet/ visible (UV/vis) spectroscopy, dynamic light scattering (DLS), and infrared (IR) spectroscopy. The presented strategy represents a versatile building block system that can be adapted to a variety of bioactive molecules and may be of high relevance for theragnostic applications.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Mattern, Annabelle UNSPECIFIED UNSPECIFIED UNSPECIFIED Habermann, Sebastian UNSPECIFIED UNSPECIFIED UNSPECIFIED Zegke, Markus UNSPECIFIED UNSPECIFIED UNSPECIFIED Wickleder, Mathias Siegfried UNSPECIFIED UNSPECIFIED UNSPECIFIED Alberto, Roger UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-667514
DOI:	10.1021/acs.bioconjchem.2c00351
Journal or Publication Title:	Bioconjugate Chem.
Volume:	33
Number:	9
Page Range:	S. 1741 - 1750
Date:	2022
Publisher:	AMER CHEMICAL SOC
Place of Publication:	WASHINGTON
ISSN:	1520-4812
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language FUNCTIONALIZATION; BIODISTRIBUTION; TECHNETIUM; DELIVERY; BINDING; DESIGN; LIVER Multiple languages Biochemical Research Methods; Biochemistry & Molecular Biology; Chemistry, Multidisciplinary; Chemistry, Organic Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/66751