Universität zu Köln

Kiefer, Daniel, Mueller-Wirtz, Lukas M., Maurer, Felix, Hueppe, Tobias, Mathes, Alexander M., Volk, Thomas ORCID: 0000-0001-5175-7159, Kreuer, Sascha and Fink, Tobias (2022). Intravenous propofol, ketamine (ketofol) and rocuronium after sevoflurane induction provides long lasting anesthesia in ventilated rats. Exp. Anim., 71 (2). S. 231 - 240. TOKYO: INT PRESS EDITING CENTRE INC. ISSN 1881-7122

Full text not available from this repository.

Abstract

Rats are commonly used animals for laboratory experiments and many experiments require general anesthesia. However, the lack of published and reproducible intravenous anesthesia protocols for rats results in unnecessary animal use to establish new anesthesia techniques across institutions. We therefore developed an anesthesia protocol with propofol, ketamine, and rocuronium for mechanically ventilated rats, and evaluated vital parameters and plasma concentrations. 15 male Sprague-Dawley rats underwent inhalation induction with sevoflurane and tracheal, venous and arterial cannulation. After established venous access, sevoflurane was substituted by propofol and ketamine (ketofol). Rocuronium was added under mechanical ventilation for 7 h. Drug dosages were stepwise reduced to prevent accumulation. All animals survived the observation period and showed adequate depth of anesthesia. Mean arterial pressure and heart rate remained within normal ranges. Median propofol plasma concentrations remained stable: 1, 4, 7 h: 2.0 (interquartile range (IQR): 1.8-2.2), 2.1 (1.8-2.2), 1.8 (1.6-2.1) pg/ml, whereas median ketamine concentrations slightly differed after 7 h compared to 1 h: 1, 4, 7 h: 3.7 (IQR: 3.5-4.5), 3.8 (3.3-4.1), 3.8 (3.0-4.1) pg/ml. Median rocuronium plasma concentrations were lower after 4 and 7 h compared to 1 h: 1, 4, 7 h: 3.9 (IQR: 3.5-4.9), 3.2 (2.7-3.3), 3.0 (2.4-3.4) pg/ml. Our anesthesia protocol provides stable and reliable anesthesia in mechanically ventilated rats for several hours.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Kiefer, Daniel UNSPECIFIED UNSPECIFIED UNSPECIFIED Mueller-Wirtz, Lukas M. UNSPECIFIED UNSPECIFIED UNSPECIFIED Maurer, Felix UNSPECIFIED UNSPECIFIED UNSPECIFIED Hueppe, Tobias UNSPECIFIED UNSPECIFIED UNSPECIFIED Mathes, Alexander M. UNSPECIFIED UNSPECIFIED UNSPECIFIED Volk, Thomas UNSPECIFIED orcid.org/0000-0001-5175-7159 UNSPECIFIED Kreuer, Sascha UNSPECIFIED UNSPECIFIED UNSPECIFIED Fink, Tobias UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-668572
DOI:	10.1538/expanim.21-0147
Journal or Publication Title:	Exp. Anim.
Volume:	71
Number:	2
Page Range:	S. 231 - 240
Date:	2022
Publisher:	INT PRESS EDITING CENTRE INC
Place of Publication:	TOKYO
ISSN:	1881-7122
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language NEUROMUSCULAR BLOCK; INJECTABLE ANESTHETICS; XYLAZINE; OXYGEN; PHARMACOKINETICS; MICROCIRCULATION; PROTOCOLS; DIAPHRAGM; MIDAZOLAM; MUSCLE Multiple languages Veterinary Sciences; Zoology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/66857