Universität zu Köln

Hamad, Sarkawt ORCID: 0000-0002-5155-9467, Derichsweiler, Daniel, Gaspar, John Antonydas, Brockmeier, Konrad, Hescheler, Juergen, Sachinidis, Agapios and Pfannkuche, Kurt Paul ORCID: 0000-0002-4284-774X (2022). High-efficient serum-free differentiation of endothelial cells from human iPS cells. Stem Cell Res. Ther., 13 (1). LONDON: BMC. ISSN 1757-6512

Full text not available from this repository.

Abstract

Introduction Endothelial cells (ECs) form the inner lining of all blood vessels of the body play important roles in vascular tone regulation, hormone secretion, anticoagulation, regulation of blood cell adhesion and immune cell extravasation. Limitless ECs sources are required to further in vitro investigations of ECs' physiology and pathophysiology as well as for tissue engineering approaches. Ideally, the differentiation protocol avoids animal-derived components such as fetal serum and yields ECs at efficiencies that make further sorting obsolete for most applications. Method Human induced pluripotent stem cells (hiPSCs) are cultured under serum-free conditions and induced into mesodermal progenitor cells via stimulation of Wnt signaling for 24 h. Mesodermal progenitor cells are further differentiated into ECs by utilizing a combination of human vascular endothelial growth factor A165 (VEGF), basic fibroblast growth factor (bFGF), 8-Bromoadenosine 3 ',5 '-cyclic monophosphate sodium salt monohydrate (8Bro) and melatonin (Mel) for 48 h. Result This combination generates hiPSC derived ECs (hiPSC-ECs) at a fraction of 90.9 +/- 1.5% and is easily transferable from the two-dimensional (2D) monolayer into three-dimensional (3D) scalable bioreactor suspension cultures. hiPSC-ECs are positive for CD31, VE-Cadherin, von Willebrand factor and CD34. Furthermore, the majority of hiPSC-ECs express the vascular endothelial marker CD184 (CXCR4). Conclusion The differentiation method presented here generates hiPSC-ECs in only 6 days, without addition of animal sera and at high efficiency, hence providing a scalable source of hiPSC-ECs.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Hamad, Sarkawt UNSPECIFIED orcid.org/0000-0002-5155-9467 UNSPECIFIED Derichsweiler, Daniel UNSPECIFIED UNSPECIFIED UNSPECIFIED Gaspar, John Antonydas UNSPECIFIED UNSPECIFIED UNSPECIFIED Brockmeier, Konrad UNSPECIFIED UNSPECIFIED UNSPECIFIED Hescheler, Juergen UNSPECIFIED UNSPECIFIED UNSPECIFIED Sachinidis, Agapios UNSPECIFIED UNSPECIFIED UNSPECIFIED Pfannkuche, Kurt Paul UNSPECIFIED orcid.org/0000-0002-4284-774X UNSPECIFIED
URN:	urn:nbn:de:hbz:38-669001
DOI:	10.1186/s13287-022-02924-x
Journal or Publication Title:	Stem Cell Res. Ther.
Volume:	13
Number:	1
Date:	2022
Publisher:	BMC
Place of Publication:	LONDON
ISSN:	1757-6512
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language PLURIPOTENT STEM-CELLS; MELATONIN; ACTIVATION; GENERATION; INDUCTION; MESODERM; GROWTH Multiple languages Cell & Tissue Engineering; Cell Biology; Medicine, Research & Experimental Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/66900