Universität zu Köln

Fang, Xinmei, Yan, Peng, Luo, Fengying, Han, Shan, Lin, Tiantian, Li, Shuying, Li, Shujiang and Zhu, Tianhui (2022). Functional Identification of Arthrinium phaeospermum Effectors Related to Bambusa pervariabilis x Dendrocalamopsis grandis Shoot Blight. Biomolecules, 12 (9). BASEL: MDPI. ISSN 2218-273X

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Abstract

The shoot blight of Bambusa pervariabilis x Dendrocalamopsis grandis caused by Arthrinium phaeospermum made bamboo die in a large area, resulting in serious ecological and economic losses. Dual RNA-seq was used to sequence and analyze the transcriptome data of A. phaeospermum and B. pervariabilis x D. grandis in the four periods after the pathogen infected the host and to screen the candidate effectors of the pathogen related to the infection. After the identification of the effectors by the tobacco transient expression system, the functions of these effectors were verified by gene knockout. Fifty-three differentially expressed candidate effectors were obtained by differential gene expression analysis and effector prediction. Among them, the effectors Delta pCE12 and Delta pCE22 can cause programmed cell death in tobacco. The disease index of B. pervariabilis x D. grandis inoculated with mutant Delta ApCE12 and mutant Delta ApCE22 strains were 52.5% and 47.5%, respectively, which was significantly lower than that of the wild-type strains (80%), the ApCE12 complementary strain (77.5%), and the ApCE22 complementary strain (75%). The tolerance of the mutant Delta ApCE12 and mutant Delta ApCE22 strains to H2O2 and NaCl stress was significantly lower than that of the wild-type strain and the ApCE12 complementary and ApCE22 complementary strains, but there was no difference in their tolerance to Congo red. Therefore, this study shows that the effectors ApCE12 and ApCE22 play an important role in A. phaeospermum virulence and response to H2O2 and NaCl stress.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Fang, Xinmei UNSPECIFIED UNSPECIFIED UNSPECIFIED Yan, Peng UNSPECIFIED UNSPECIFIED UNSPECIFIED Luo, Fengying UNSPECIFIED UNSPECIFIED UNSPECIFIED Han, Shan UNSPECIFIED UNSPECIFIED UNSPECIFIED Lin, Tiantian UNSPECIFIED UNSPECIFIED UNSPECIFIED Li, Shuying UNSPECIFIED UNSPECIFIED UNSPECIFIED Li, Shujiang UNSPECIFIED UNSPECIFIED UNSPECIFIED Zhu, Tianhui UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-670611
DOI:	10.3390/biom12091264
Journal or Publication Title:	Biomolecules
Volume:	12
Number:	9
Date:	2022
Publisher:	MDPI
Place of Publication:	BASEL
ISSN:	2218-273X
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language RNA-SEQ ANALYSIS; TRANSCRIPTION FACTORS; PROTEIN; PATHOGEN; EXPRESSION; ETHYLENE; DEFENSE; KINASE; TOMATO; PURIFICATION Multiple languages Biochemistry & Molecular Biology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/67061