Universität zu Köln

Solheim, Sara Amalie, Thomas, Andreas, Ringsted, Thomas Kamm, Thevis, Mario, Breenfeldt Andersen, Andreas, Holm-Sorensen, Henrik, Nordsborg, Nikolai B. and Morkeberg, Jakob (2022). Analysis of dried blood spots is a feasible alternative for detecting ephedrine in doping control. Drug Test. Anal., 14 (10). S. 1685 - 1696. HOBOKEN: WILEY. ISSN 1942-7611

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Abstract

Dried blood spot (DBS) testing allows fast, easy and minimally invasive collection of microvolumes of blood. In an anti-doping context, DBS testing has particular relevance for substances prohibited in-competition only such as ephedrine, which is currently detected by urine analysis, because DBS can add information about the blood drug concentrations during the in-competition period. Several collection methods and devices exist for DBS collection from different anatomical sites. Thus, agreements between concentrations of target analytes in DBS samples from different sampling sites, along with between DBS and those in conventional venous plasma samples, need to be evaluated. Herein, we collected matched upper-arm DBS, fingerprick DBS and venous plasma samples from eight healthy male subjects in an 8-h period following oral administrations of 20 mg ('low dose') and 60 mg ('high dose') of ephedrine. We show that the use of alternative sampling sites and matrices is a feasible possibility for ephedrine analysis in doping control. We observed very good agreement between collection sites and that specificity and sensitivity can be upheld despite use of an alternative collection site. However, potential concentration differences between DBS and venous plasma should be considered, and distinct threshold might be necessary if implementing both blood matrices in ephedrine analysis.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Solheim, Sara Amalie UNSPECIFIED UNSPECIFIED UNSPECIFIED Thomas, Andreas UNSPECIFIED UNSPECIFIED UNSPECIFIED Ringsted, Thomas Kamm UNSPECIFIED UNSPECIFIED UNSPECIFIED Thevis, Mario UNSPECIFIED UNSPECIFIED UNSPECIFIED Breenfeldt Andersen, Andreas UNSPECIFIED UNSPECIFIED UNSPECIFIED Holm-Sorensen, Henrik UNSPECIFIED UNSPECIFIED UNSPECIFIED Nordsborg, Nikolai B. UNSPECIFIED UNSPECIFIED UNSPECIFIED Morkeberg, Jakob UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-671394
DOI:	10.1002/dta.3338
Journal or Publication Title:	Drug Test. Anal.
Volume:	14
Number:	10
Page Range:	S. 1685 - 1696
Date:	2022
Publisher:	WILEY
Place of Publication:	HOBOKEN
ISSN:	1942-7611
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language DRUG CONCENTRATION; MARKED DEPENDENCE; SAMPLING SITE; CAPILLARY; PHARMACOKINETICS; PHARMACODYNAMICS; TOXICOLOGY; RATIONALE; CAFFEINE; DBS Multiple languages Biochemical Research Methods; Chemistry, Analytical; Pharmacology & Pharmacy Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/67139