Universität zu Köln

Ruiter, Floor A. A., Morgan, Francis L. C., Roumans, Nadia, Schumacher, Anika, Slaats, Gisela G., Moroni, Lorenzo ORCID: 0000-0003-1298-6025, LaPointe, Vanessa L. S. and Baker, Matthew B. (2022). Soft, Dynamic Hydrogel Confinement Improves Kidney Organoid Lumen Morphology and Reduces Epithelial-Mesenchymal Transition in Culture. Adv. Sci., 9 (20). HOBOKEN: WILEY. ISSN 2198-3844

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Abstract

Pluripotent stem cell-derived kidney organoids offer a promising solution to renal failure, yet current organoid protocols often lead to off-target cells and phenotypic alterations, preventing maturity. Here, various dynamic hydrogel architectures are created, conferring a controlled and biomimetic environment for organoid encapsulation. How hydrogel stiffness and stress relaxation affect renal phenotype and undesired fibrotic markers are investigated. The authors observe that stiff hydrogel encapsulation leads to an absence of certain renal cell types and signs of an epithelial-mesenchymal transition (EMT), whereas encapsulation in soft, stress-relaxing hydrogels leads to all major renal segments, fewer fibrosis or EMT associated proteins, apical proximal tubule polarization, and primary cilia formation, representing a significant improvement over current approaches to culture kidney organoids. The findings show that engineering hydrogel mechanics and dynamics have a decided benefit for organoid culture. These structure-property-function relationships can enable the rational design of materials, bringing us closer to functional engraftments and disease-modeling applications.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Ruiter, Floor A. A. UNSPECIFIED UNSPECIFIED UNSPECIFIED Morgan, Francis L. C. UNSPECIFIED UNSPECIFIED UNSPECIFIED Roumans, Nadia UNSPECIFIED UNSPECIFIED UNSPECIFIED Schumacher, Anika UNSPECIFIED UNSPECIFIED UNSPECIFIED Slaats, Gisela G. UNSPECIFIED UNSPECIFIED UNSPECIFIED Moroni, Lorenzo UNSPECIFIED orcid.org/0000-0003-1298-6025 UNSPECIFIED LaPointe, Vanessa L. S. UNSPECIFIED UNSPECIFIED UNSPECIFIED Baker, Matthew B. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-671405
DOI:	10.1002/advs.202200543
Journal or Publication Title:	Adv. Sci.
Volume:	9
Number:	20
Date:	2022
Publisher:	WILEY
Place of Publication:	HOBOKEN
ISSN:	2198-3844
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language REGENERATIVE MEDICINE; PRIMARY CILIA; CELLS; GENERATION; MORPHOGENESIS; INJURY Multiple languages Chemistry, Multidisciplinary; Nanoscience & Nanotechnology; Materials Science, Multidisciplinary Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/67140