Universität zu Köln

Engberg, Oskar ORCID: 0000-0002-0284-5504, Ulbricht, David, Dobel, Viola, Siebert, Verena ORCID: 0000-0002-6951-6887, Frie, Christian, Penk, Anja, Lemberg, Marius K. and Huster, Daniel (2022). Rhomboid-catalyzed intramembrane proteolysis requires hydrophobic matching with the surrounding lipid bilayer. Sci. Adv., 8 (38). WASHINGTON: AMER ASSOC ADVANCEMENT SCIENCE. ISSN 2375-2548

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Abstract

Membrane thinning by rhomboid proteins has been proposed to reduce hydrophobic mismatch, providing a unique environment for important functions ranging from intramembrane proteolysis to retrotranslocation in protein degradation. We show by in vitro reconstitution and solid-state nuclear magnetic resonance that the lipid environment of the Escherichia coli rhomboid protease GlpG influences its activity with an optimal hydrophobic membrane thickness between 24 and 26 angstrom. While phosphatidylcholine membranes are only negligibly altered by GlpG, in an E. coli-relevant lipid mix of phosphatidylethanolamine and phosphatidylglycerol, a thinning by 1.1 angstrom per leaflet is observed. Protease activity is strongly correlated with membrane thickness and shows no lipid headgroup specificity. We infer from these results that, by adjusting the thickness of specific membrane domains, membrane proteins shape the bilayer for their specific needs.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Engberg, Oskar UNSPECIFIED orcid.org/0000-0002-0284-5504 UNSPECIFIED Ulbricht, David UNSPECIFIED UNSPECIFIED UNSPECIFIED Dobel, Viola UNSPECIFIED UNSPECIFIED UNSPECIFIED Siebert, Verena UNSPECIFIED orcid.org/0000-0002-6951-6887 UNSPECIFIED Frie, Christian UNSPECIFIED UNSPECIFIED UNSPECIFIED Penk, Anja UNSPECIFIED UNSPECIFIED UNSPECIFIED Lemberg, Marius K. UNSPECIFIED UNSPECIFIED UNSPECIFIED Huster, Daniel UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-673174
DOI:	10.1126/sciadv.abq8303
Journal or Publication Title:	Sci. Adv.
Volume:	8
Number:	38
Date:	2022
Publisher:	AMER ASSOC ADVANCEMENT SCIENCE
Place of Publication:	WASHINGTON
ISSN:	2375-2548
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language NMR-SPECTROSCOPY; GAMMA-SECRETASE; MEMBRANE; PROTEIN; PEPTIDE; ORGANIZATION; CHOLESTEROL; MISMATCH; REVEALS Multiple languages Multidisciplinary Sciences Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/67317