Universität zu Köln

Wendel, Andreas F. ORCID: 0000-0002-2160-4220, Malecki, Monika, Mattner, Frauke, Xanthopoulou, Kyriaki ORCID: 0000-0001-8591-8184, Wille, Julia, Seifert, Harald and Higgins, Paul G. (2022). Genomic-based transmission analysis of carbapenem-resistant Pseudomonas aeruginosa at a tertiary care centre in Cologne (Germany) from 2015 to 2020. JAC-Antimicrob. Resist., 4 (3). OXFORD: OXFORD UNIV PRESS. ISSN 2632-1823

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Abstract

Objectives To describe the propensity of carbapenem-resistant Pseudomonas aeruginosa to spread within a hospital critical care setting. Methods The study was conducted in a 700-bed tertiary centre in Cologne, Germany. P. aeruginosa resistant to piperacillin, ceftazidime, cefepime, imipenem, meropenem and ciprofloxacin, isolated from clinical and screening specimens from four critical care units from 2015 to 2020 were analysed. Genotyping was carried out by WGS (Illumina and MinION). MLST, core genome MLST (cgMLST) and resistome analysis was performed and merged with epidemiological data. Results Fifty-five out of 79 non-duplicate P. aeruginosa isolates were available, of which 20 were carbapenemase producers as follows: bla(VIM-1) (n = 1), bla(VIM-2) (n = 17), bla(VIM-4) (n = 1), and bla(NDM-1)/bla(GES-5) (n = 1). Forty-two of 55 isolates were hospital-acquired. cgMLST revealed three clusters: Cluster 1 (n = 15, ST111, bla(VIM-2), recovered between 2015 and 2020); Cluster 2 (n = 4, ST970, carbapenemase negative); and Cluster 3 (n = 2, ST357, carbapenemase negative). The bla(VIM-2) gene of Cluster 1 was integrated on the chromosome in a class 1 integron (type In59). Using conventional epidemiology, we were only able to confirm two patient-to-patient transmissions and one room-to-patient transmission on three different ICUs within Cluster 1. Isolates from Cluster 2 represented an outbreak occurring in 2019. Conclusions These data give insight into the epidemiology of carbapenem-resistant P. aeruginosa. Transmission dynamics differed between carbapenemase- and non-carbapenemase-producing isolates. A continuous acquisition of clonally related ST111 VIM-2 P. aeruginosa, being the main carbapenemase-producing strain, was observed over the whole study period, as well as an overall higher genomic diversity among non-carbapenemase-producing P. aeruginosa.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Wendel, Andreas F. UNSPECIFIED orcid.org/0000-0002-2160-4220 UNSPECIFIED Malecki, Monika UNSPECIFIED UNSPECIFIED UNSPECIFIED Mattner, Frauke UNSPECIFIED UNSPECIFIED UNSPECIFIED Xanthopoulou, Kyriaki UNSPECIFIED orcid.org/0000-0001-8591-8184 UNSPECIFIED Wille, Julia UNSPECIFIED UNSPECIFIED UNSPECIFIED Seifert, Harald UNSPECIFIED UNSPECIFIED UNSPECIFIED Higgins, Paul G. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-673409
DOI:	10.1093/jacamr/dlac057
Journal or Publication Title:	JAC-Antimicrob. Resist.
Volume:	4
Number:	3
Date:	2022
Publisher:	OXFORD UNIV PRESS
Place of Publication:	OXFORD
ISSN:	2632-1823
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language OUTBREAK; INFECTIONS; EPIDEMIOLOGY; ST111 Multiple languages Infectious Diseases; Microbiology; Pharmacology & Pharmacy Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/67340