Universität zu Köln

Zweerink, Susanne, Mueck, Vera, Kraemer, Laurenz P., Tischler, Eva, Schulte, Sigrid, Goeser, Tobias and Nierhoff, Dirk (2022). Repolarization Precedes Oval Cell-mediated Hepatocytic Regeneration in the CDE Diet Mouse Model. J. Histochem. Cytochem., 70 (5). S. 377 - 390. LONDON: SAGE PUBLICATIONS LTD. ISSN 1551-5044

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Abstract

The liver has a unique ability to recover from injury unlike any other organ. A poorly understood aspect of liver regeneration is the role of hepatocellular polarization. Neighbor of Punc E11 (Nope) is an oncofetal stem/progenitor cell marker, which is expressed by depolarized adult hepatocytes after cholestatic liver injury and in hepatocellular carcinoma. Liver injury induced by a choline-deficient and ethionine-supplemented diet is reversible if followed by an additional dietary stop interval and enabled us to study the expression of Nope during the induction of chronic liver injury and during subsequent liver regeneration. We could show by quantitative RT-PCR, Western blotting, and immunohistochemistry that the expression of Nope is induced in depolarized adult hepatocytes during injury. However, after another 2 weeks of a normal diet, the polarization of hepatocytes was almost completely restored and the expression of Nope remained limited to bile ducts and oval cells. Using an inducible CK19-lineage tracing model, we could demonstrate that oval cell-mediated hepatocyte regeneration is rare and was preceded by repolarization of hepatocytes. In conclusion, polarization of hepatocytes is an important part of liver regeneration and precedes oval cell-mediated regeneration of the liver. This process can be visualized by a characteristic expression pattern of Nope:

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Zweerink, Susanne UNSPECIFIED UNSPECIFIED UNSPECIFIED Mueck, Vera UNSPECIFIED UNSPECIFIED UNSPECIFIED Kraemer, Laurenz P. UNSPECIFIED UNSPECIFIED UNSPECIFIED Tischler, Eva UNSPECIFIED UNSPECIFIED UNSPECIFIED Schulte, Sigrid UNSPECIFIED UNSPECIFIED UNSPECIFIED Goeser, Tobias UNSPECIFIED UNSPECIFIED UNSPECIFIED Nierhoff, Dirk UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-673627
DOI:	10.1369/00221554221084665
Journal or Publication Title:	J. Histochem. Cytochem.
Volume:	70
Number:	5
Page Range:	S. 377 - 390
Date:	2022
Publisher:	SAGE PUBLICATIONS LTD
Place of Publication:	LONDON
ISSN:	1551-5044
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language PROGENITOR CELLS; LIVER-INJURY; PUNC E11; EXPRESSION; NEIGHBOR; MARKER; FETAL; CHOLANGIOCYTES; GENE; NOPE Multiple languages Cell Biology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/67362