Universität zu Köln

Chockley, Alexander S., Dinges, Gesa F. ORCID: 0000-0002-1759-738X, Di Cristina, Giulia ORCID: 0000-0001-6253-4807, Ratican, Sara ORCID: 0000-0002-7864-0070, Bockemuehl, Till and Bueschges, Ansgar (2022). Subsets of leg proprioceptors influence leg kinematics but not interleg coordination in Drosophila melanogaster walking. J. Exp. Biol., 225 (20). CAMBRIDGE: COMPANY BIOLOGISTS LTD. ISSN 1477-9145

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Abstract

Legged locomotion in terrestrial animals is often essential for mating and survival, and locomotor behavior must be robust and adaptable to be successful. This adaptability is largely provided by proprioceptors monitoring positions and movements of body parts and providing feedback to other components of locomotor networks. In insects, proprioceptive chordotonal organs span joints and encode parameters of relative movement between segments. Previous studies have used whole-organ ablation, reduced preparations or broad physiological manipulations to impair the function of the femoral chordotonal organ ( fCO), which monitors the femur-tibia joint, and have demonstrated its contribution to interleg coordination and walking behavior. The fCO in Drosophila melanogaster comprises groups of neurons that differ in their morphology and encoding properties (club, hook, claw); sub-population-level manipulations of fCO function have not been methodologically accessible. Here, we took advantage of the genetic toolkit available in D. melanogaster to identify sub-populations of fCO neurons and used transient optogenetic inhibition to investigate their roles in locomotor coordination. Our findings demonstrate that optogenetic inhibition of a subset of club and hook neurons replicates the effects of inhibiting the whole fCO; when inhibited alone, however, the individual subset types did not strongly affect spatial aspects of single-leg kinematics. Moreover, fCO subsets seem to play only a minor role in interleg temporal coordination. Thus, the fCO contains functionally distinct subgroups, and this functional classification may differ from those based on anatomy and encoding properties; this should be investigated in future studies of proprioceptors and their involvement in locomotor networks.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Chockley, Alexander S. UNSPECIFIED UNSPECIFIED UNSPECIFIED Dinges, Gesa F. UNSPECIFIED orcid.org/0000-0002-1759-738X UNSPECIFIED Di Cristina, Giulia UNSPECIFIED orcid.org/0000-0001-6253-4807 UNSPECIFIED Ratican, Sara UNSPECIFIED orcid.org/0000-0002-7864-0070 UNSPECIFIED Bockemuehl, Till UNSPECIFIED UNSPECIFIED UNSPECIFIED Bueschges, Ansgar UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-674581
DOI:	10.1242/jeb.244245
Journal or Publication Title:	J. Exp. Biol.
Volume:	225
Number:	20
Date:	2022
Publisher:	COMPANY BIOLOGISTS LTD
Place of Publication:	CAMBRIDGE
ISSN:	1477-9145
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language FEMORAL CHORDOTONAL ORGAN; TIBIA CONTROL-SYSTEM; STICK INSECT; SENSORY FEEDBACK; MOTOR CONTROL; PHYSIOLOGY; MOVEMENTS; PROJECTIONS; NEURONS; JOINT Multiple languages Biology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/67458