Universität zu Köln

Andersson, Alexander ORCID: 0000-0001-6498-2603, Piper, Thomas, Ekstrom, Lena ORCID: 0000-0003-1053-2345, Hirschberg, Angelica Linden ORCID: 0000-0001-6481-6277 and Thevis, Mario (2023). Usefulness of serum androgen isotope ratio mass spectrometry (IRMS) to detect testosterone supplementation in women. Drug Test. Anal., 15 (4). S. 465 - 470. HOBOKEN: WILEY. ISSN 1942-7611

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Abstract

The detection of testosterone intake is facilitated by monitoring the urinary steroid profile in the athlete biological passport. This technique can be used with confidence to identify target samples for isotope ratio mass spectrometry. Regrettably, most research has been performed on male subjects resulting in a method that does not account for females' steroid concentration and/or variation. This study evaluates the usefulness of the carbon isotope ratio (CIR) in serum of female subjects. Two steroid sulphates are targeted in serum, androsterone and epiandrosterone. Both exhibit statistically significant depletion of their CIR after 10 weeks of daily (10 mg) transdermal testosterone administration. Of the 21 female subjects, samples from six individuals were identified as adverse analytical findings; additionally, four were found atypical considering the serum CIR. The urinary athlete biological passport was not sufficiently sensitive to identify target serum samples for isotope ratio mass spectroscopy. Of the six with a suspicious passport, only two could be confirmed using the serum CIR of androsterone and epiandrosterone. This study shows that CIR analysis in serum cannot be considered the sole confirmatory solution to detect testosterone doping in women due to low sensitivity. However, this analysis has the potential to be used as a complementary method in certain situations to confirm exogenous testosterone in women.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Andersson, Alexander UNSPECIFIED orcid.org/0000-0001-6498-2603 UNSPECIFIED Piper, Thomas UNSPECIFIED UNSPECIFIED UNSPECIFIED Ekstrom, Lena UNSPECIFIED orcid.org/0000-0003-1053-2345 UNSPECIFIED Hirschberg, Angelica Linden UNSPECIFIED orcid.org/0000-0001-6481-6277 UNSPECIFIED Thevis, Mario UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-674736
DOI:	10.1002/dta.3428
Journal or Publication Title:	Drug Test. Anal.
Volume:	15
Number:	4
Page Range:	S. 465 - 470
Date:	2023
Publisher:	WILEY
Place of Publication:	HOBOKEN
ISSN:	1942-7611
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language STEROID PROFILE; METABOLISM; ABUSE Multiple languages Biochemical Research Methods; Chemistry, Analytical; Pharmacology & Pharmacy Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/67473