Universität zu Köln

Tietgen, Manuela, Sedlaczek, Lisa, Higgins, Paul G. G., Kaspar, Heike, Ewers, Christa ORCID: 0000-0001-6167-1340 and Goettig, Stephan (2022). Colistin Resistance Mechanisms in Human and Veterinary Klebsiella pneumoniae Isolates. Antibiotics-Basel, 11 (11). BASEL: MDPI. ISSN 2079-6382

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Abstract

Colistin (polymyxin E) is increasingly used as a last-resort antibiotic for the treatment of severe infections with multidrug-resistant Gram-negative bacteria. In contrast to human medicine, colistin is also used in veterinary medicine for metaphylaxis. Our objective was to decipher common colistin resistance mechanisms in Klebsiella pneumoniae isolates from animals. In total, 276 veterinary K. pneumoniae isolates, derived from companion animals or livestock, and 12 isolates from human patients were included for comparison. Six out of 276 veterinary isolates were colistin resistant (2.2%). Human isolates belonging to high-risk clonal lineages (e.g., ST15, ST101, ST258), displayed multidrug-resistant phenotypes and harboured many resistance genes compared to the veterinary isolates. However, the common colistin resistance mechanism in both human and animal K. pneumoniae isolates were diverse alterations of MgrB, a critical regulator of lipid A modification. Additionally, deleterious variations of lipopolysaccharide (LPS)-associated proteins (e.g., PmrB P95L, PmrE P89L, LpxB A152T) were identified. Phylogenetic analysis and mutation patterns in genes encoding LPS-associated proteins indicated that colistin resistance mechanisms developed independently in human and animal isolates. Since only very few antibiotics remain to treat infections with MDR bacteria, it is important to further analyse resistance mechanisms and the dissemination within different isolates and sources.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Tietgen, Manuela UNSPECIFIED UNSPECIFIED UNSPECIFIED Sedlaczek, Lisa UNSPECIFIED UNSPECIFIED UNSPECIFIED Higgins, Paul G. G. UNSPECIFIED UNSPECIFIED UNSPECIFIED Kaspar, Heike UNSPECIFIED UNSPECIFIED UNSPECIFIED Ewers, Christa UNSPECIFIED orcid.org/0000-0001-6167-1340 UNSPECIFIED Goettig, Stephan UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-674750
DOI:	10.3390/antibiotics11111672
Journal or Publication Title:	Antibiotics-Basel
Volume:	11
Number:	11
Date:	2022
Publisher:	MDPI
Place of Publication:	BASEL
ISSN:	2079-6382
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language CATIONIC ANTIMICROBIAL PEPTIDES; PSEUDOMONAS-AERUGINOSA; POLYMYXIN RESISTANCE; BETA-LACTAMASES; MUTATIONS; EMERGENCE; EPIDEMIOLOGY; INACTIVATION; MEMBRANE; ANIMALS Multiple languages Infectious Diseases; Pharmacology & Pharmacy Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/67475