Universität zu Köln

Knapp, Elizabeth M., Kaiser, Andrea, Arnold, Rebecca C., Sampson, Maureen M., Ruppert, Manuela, Xu, Li, Anderson, Matthew, I, Bonanno, Shivan L., Scholz, Henrike ORCID: 0000-0001-8619-5328, Donlea, Jeffrey M. and Krantz, David E. (2022). Mutation of the Drosophila melanogaster serotonin transporter dSERT impacts sleep, courtship, and feeding behaviors. PLoS Genet., 18 (11). SAN FRANCISCO: PUBLIC LIBRARY SCIENCE. ISSN 1553-7404

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Abstract

The Serotonin Transporter (SERT) regulates extracellular serotonin levels and is the target of most current drugs used to treat depression. The mechanisms by which inhibition of SERT activity influences behavior are poorly understood. To address this question in the model organism Drosophila melanogaster, we developed new loss of function mutations in Drosophila SERT (dSERT). Previous studies in both flies and mammals have implicated serotonin as an important neuromodulator of sleep, and our newly generated dSERT mutants show an increase in total sleep and altered sleep architecture that is mimicked by feeding the SSRI citalopram. Differences in daytime versus nighttime sleep architecture as well as genetic rescue experiments unexpectedly suggest that distinct serotonergic circuits may modulate daytime versus nighttime sleep. dSERT mutants also show defects in copulation and food intake, akin to the clinical side effects of SSRIs and consistent with the pleomorphic influence of serotonin on the behavior of D. melanogaster. Starvation did not overcome the sleep drive in the mutants and in male dSERT mutants, the drive to mate also failed to overcome sleep drive. dSERT may be used to further explore the mechanisms by which serotonin regulates sleep and its interplay with other complex behaviors. Author summary Many medications used to treat depression and anxiety act by changing serotonin levels in the brain. Fruit flies also use serotonin and can be used as a model to study the brain. We have made a fly mutant for the serotonin transporter (SERT), which is the target of antidepressants in humans. The mutants sleep more, eat less, and have a decreased sex drive. These flies can be used to study the neuronal pathways by which serotonin regulates sleep, eating and sexual behaviors and may help us to understand the behavioral effects of antidepressants.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Knapp, Elizabeth M. UNSPECIFIED UNSPECIFIED UNSPECIFIED Kaiser, Andrea UNSPECIFIED UNSPECIFIED UNSPECIFIED Arnold, Rebecca C. UNSPECIFIED UNSPECIFIED UNSPECIFIED Sampson, Maureen M. UNSPECIFIED UNSPECIFIED UNSPECIFIED Ruppert, Manuela UNSPECIFIED UNSPECIFIED UNSPECIFIED Xu, Li UNSPECIFIED UNSPECIFIED UNSPECIFIED Anderson, Matthew, I UNSPECIFIED UNSPECIFIED UNSPECIFIED Bonanno, Shivan L. UNSPECIFIED UNSPECIFIED UNSPECIFIED Scholz, Henrike UNSPECIFIED orcid.org/0000-0001-8619-5328 UNSPECIFIED Donlea, Jeffrey M. UNSPECIFIED UNSPECIFIED UNSPECIFIED Krantz, David E. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-675598
DOI:	10.1371/journal.pgen.1010289
Journal or Publication Title:	PLoS Genet.
Volume:	18
Number:	11
Date:	2022
Publisher:	PUBLIC LIBRARY SCIENCE
Place of Publication:	SAN FRANCISCO
ISSN:	1553-7404
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language PARADOXICAL SLEEP; BIOGENIC-AMINES; NEURONS; GENE; POLYMORPHISM; RECEPTORS; MUTANTS; ABNORMALITIES; PERSPECTIVES; DYSFUNCTION Multiple languages Genetics & Heredity Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/67559