Universität zu Köln

Lukacisin, Martin ORCID: 0000-0001-6549-4177, Espinosa-Cantu, Adriana and Bollenbach, Tobias ORCID: 0000-0003-4398-476X (2022). Intron-mediated induction of phenotypic heterogeneity. Nature, 605 (7908). S. 113 - 137. BERLIN: NATURE PORTFOLIO. ISSN 1476-4687

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Abstract

Intragenic regions that are removed during maturation of the RNA transcriptintrons-are universally present in the nuclear genomes of eukaryotes(1). The budding yeast, an otherwise intron-poor species, preserves two sets of ribosomal protein genes that differ primarily in their introns(2,3). Although studies have shed light on the role of ribosomal protein introns under stress and starvation(4-6), understanding the contribution of introns to ribosome regulation remains challenging. Here, by combining isogrowth profiling7 with single-cell protein measurements(8), we show that introns can mediate inducible phenotypic heterogeneity that confers a clear fitness advantage. Osmotic stress leads to bimodal expression of the small ribosomal subunit protein Rps22B, which is mediated by an intron in the 5' untranslated region of its transcript. The two resulting yeast subpopulations differ in their ability to cope with starvation. Low levels of Rps22B protein result in prolonged survival under sustained starvation, whereas high levels of Rps22B enable cells to grow faster after transient starvation. Furthermore, yeasts growing at high concentrations of sugar, similar to those in ripe grapes, exhibit bimodal expression of Rps22B when approaching the stationary phase. Differential intron-mediated regulation of ribosomal protein genes thus provides a way to diversify the population when starvation threatens in natural environments. Our findings reveal a role for introns in inducing phenotypic heterogeneity in changing environments, and suggest that duplicated ribosomal protein genes in yeast contribute to resolving the evolutionary conflict between precise expression control and environmental responsiveness(9).

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Lukacisin, Martin UNSPECIFIED orcid.org/0000-0001-6549-4177 UNSPECIFIED Espinosa-Cantu, Adriana UNSPECIFIED UNSPECIFIED UNSPECIFIED Bollenbach, Tobias UNSPECIFIED orcid.org/0000-0003-4398-476X UNSPECIFIED
URN:	urn:nbn:de:hbz:38-675650
DOI:	10.1038/s41586-022-04633-0
Journal or Publication Title:	Nature
Volume:	605
Number:	7908
Page Range:	S. 113 - 137
Date:	2022
Publisher:	NATURE PORTFOLIO
Place of Publication:	BERLIN
ISSN:	1476-4687
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language RIBOSOMAL-PROTEIN GENES; GENOME DUPLICATION; GROWTH; EVOLUTION; VISUALIZATION; SELECTION; ALIGNMENT; SEARCH Multiple languages Multidisciplinary Sciences Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/67565