Universität zu Köln

Jacobs, Chris G. C., van der Hulst, Remy, Chen, Yen-Ta, Williamson, Ryan P., Roth, Siegfried ORCID: 0000-0001-5772-3558 and van der Zee, Maurijn (2022). Immune function of the serosa in hemimetabolous insect eggs. Philos. Trans. R. Soc. B-Biol. Sci., 377 (1865). LONDON: ROYAL SOC. ISSN 1471-2970

Full text not available from this repository.

Abstract

Insects comprise more than a million species and many authors have attempted to explain this success by evolutionary innovations. A much overlooked evolutionary novelty of insects is the serosa, an extraembryonic epithelium around the yolk and embryo. We have shown previously that this epithelium provides innate immune protection to eggs of the beetle Tribolium castaneum. It remained elusive, however, whether this immune competence evolved in the Tribolium lineage or is ancestral to all insects. Here, we expand our studies to two hemimetabolous insects, the bug Oncopeltus fasciatus and the swarming grasshopper Locusta migratoria. For Oncopeltus, RNA sequencing reveals an extensive response upon infection, including the massive upregulation of antimicrobial peptides (AMPs). We demonstrate antimicrobial activity of these peptides using in vitro bacterial growth assays and describe two novel AMP families called Serosins and Ovicins. For both insects, quantitative polymerase chain reaction shows immune competence of the eggs when the serosa is present, and in situ hybridizations demonstrate that immune gene expression is localized in the serosa. This first evidence from hemimetabolous insect eggs suggests that immune competence is an ancestral property of the serosa. The evolutionary origin of the serosa with its immune function might have facilitated the spectacular radiation of the insects.This article is part of the theme issue 'Extraembryonic tissues: exploring concepts, definitions and functions across the animal kingdom'.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Jacobs, Chris G. C. UNSPECIFIED UNSPECIFIED UNSPECIFIED van der Hulst, Remy UNSPECIFIED UNSPECIFIED UNSPECIFIED Chen, Yen-Ta UNSPECIFIED UNSPECIFIED UNSPECIFIED Williamson, Ryan P. UNSPECIFIED UNSPECIFIED UNSPECIFIED Roth, Siegfried UNSPECIFIED orcid.org/0000-0001-5772-3558 UNSPECIFIED van der Zee, Maurijn UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-676204
DOI:	10.1098/rstb.2021.0266
Journal or Publication Title:	Philos. Trans. R. Soc. B-Biol. Sci.
Volume:	377
Number:	1865
Date:	2022
Publisher:	ROYAL SOC
Place of Publication:	LONDON
ISSN:	1471-2970
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language MATERNAL TRANSFER; EXPRESSION; DROSOPHILA; DEFENSE; GENE; AMNIOSEROSA; BACTERIAL; RESPONSES; IDENTITY; DATABASE Multiple languages Biology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/67620